Document Detail

Morphology of mitochondrial nucleoids, mitochondria, and nuclei during meiosis and sporulation of the yeast Saccharomycodes ludwigii.
MedLine Citation:
PMID:  22449749     Owner:  NLM     Status:  MEDLINE    
The morphology of mitochondrial nucleoids (mt-nucleoids), mitochondria, and nuclei was investigated during meiosis and sporulation of the diploid cells of the ascosporogenic yeast Saccharomycodes ludwigii. The mt-nucleoids appeared as discrete dots uniformly distributed in stationary-phase cells as revealed by 4',6-diamidino-2-phenylindole (DAPI) staining. Throughout first and second meiotic divisions, the mt-nucleoids moved to be located close to the dividing nuclei with the appearance of dots. On the other hand, mitochondria, which had tubular or fragmented forms in stationary-phase cells, increasingly fused with each other to form elongated mitochondria during meiotic prophase as revealed by 3,3' -dihexyloxacarbocyanine iodide [DiOC(6)(3)] staining. Mitochondria assembled to be located close to dividing nuclei during first and second meiotic divisions, and were finally incorporated into spores. During the first meiotic division, nuclear division occurred in any direction parallel, diagonally, or perpendicular to the longitudinal axis of the cell. In contrast, the second meiotic division was exclusively parallel to the longitudinal axis of the cell. The behavior of dividing nuclei explains the formation of a pair of spores with opposite mating types at both ends of cells. In the course of this study, it was also found that ledges between two spores were specifically stained with DiOC(6)(3).
Isamu Miyakawa; Ayumi Nakahara; Kohei Ito
Related Documents :
7852279 - Coordinate modulation of melanogenesis and type i trimer collagen secretion by type i c...
19379669 - Epilysin (mmp-28) is deposited to the basolateral extracellular matrix of epithelial ce...
15039999 - From cell-ecm interactions to tissue engineering.
12117109 - Characterisation of reconstituted equine cartilage formed in vitro.
20927339 - Quantification of three-dimensional cell-mediated collagen remodeling using graph theory.
900519 - Enzymatic isolation of osteoblasts from fetal rat calvaria.
25077289 - Timing and mechanism of the initial cue establishing handed left–right asymmetry in c...
21486179 - Amelogenin enhances the proliferation of cementoblast lineage cells.
21725609 - Prl-3 promotes the proliferation of lovo cells via the upregulation of kcnn4 channels.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of general and applied microbiology     Volume:  58     ISSN:  1349-8037     ISO Abbreviation:  J. Gen. Appl. Microbiol.     Publication Date:  2012  
Date Detail:
Created Date:  2012-03-27     Completed Date:  2012-07-12     Revised Date:  2012-08-17    
Medline Journal Info:
Nlm Unique ID:  0165543     Medline TA:  J Gen Appl Microbiol     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  43-51     Citation Subset:  IM    
Department of Environmental Science and Engineering, Graduate School of Science and Engineering, Yamaguchi University, Yamaguchi 753-8512, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Carbocyanines / metabolism
Cell Nucleus / genetics,  metabolism
Cell Nucleus Shape*
Cell Wall / metabolism
Culture Media / metabolism
Genome, Mitochondrial*
Indoles / metabolism
Microscopy, Fluorescence
Microscopy, Phase-Contrast
Mitochondria / genetics*,  metabolism,  physiology
Saccharomycetales / genetics*,  metabolism,  physiology
Spores, Fungal / genetics,  metabolism,  physiology*
Staining and Labeling
Reg. No./Substance:
0/Carbocyanines; 0/Culture Media; 0/Indoles; 47165-04-8/DAPI; 54501-79-0/3,3'-dihexyl-2,2'-oxacarbocyanine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Identification and characterization of filamentous fungi isolated from fermentation starters for Hon...
Next Document:  Cerebrospinal Fluid Substance P-Like Immunoreactivity Correlates with Aggression in Personality Diso...