Document Detail


Morphology of mitochondrial nucleoids, mitochondria, and nuclei during meiosis and sporulation of the yeast Saccharomycodes ludwigii.
MedLine Citation:
PMID:  22449749     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The morphology of mitochondrial nucleoids (mt-nucleoids), mitochondria, and nuclei was investigated during meiosis and sporulation of the diploid cells of the ascosporogenic yeast Saccharomycodes ludwigii. The mt-nucleoids appeared as discrete dots uniformly distributed in stationary-phase cells as revealed by 4',6-diamidino-2-phenylindole (DAPI) staining. Throughout first and second meiotic divisions, the mt-nucleoids moved to be located close to the dividing nuclei with the appearance of dots. On the other hand, mitochondria, which had tubular or fragmented forms in stationary-phase cells, increasingly fused with each other to form elongated mitochondria during meiotic prophase as revealed by 3,3' -dihexyloxacarbocyanine iodide [DiOC(6)(3)] staining. Mitochondria assembled to be located close to dividing nuclei during first and second meiotic divisions, and were finally incorporated into spores. During the first meiotic division, nuclear division occurred in any direction parallel, diagonally, or perpendicular to the longitudinal axis of the cell. In contrast, the second meiotic division was exclusively parallel to the longitudinal axis of the cell. The behavior of dividing nuclei explains the formation of a pair of spores with opposite mating types at both ends of cells. In the course of this study, it was also found that ledges between two spores were specifically stained with DiOC(6)(3).
Authors:
Isamu Miyakawa; Ayumi Nakahara; Kohei Ito
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of general and applied microbiology     Volume:  58     ISSN:  1349-8037     ISO Abbreviation:  J. Gen. Appl. Microbiol.     Publication Date:  2012  
Date Detail:
Created Date:  2012-03-27     Completed Date:  2012-07-12     Revised Date:  2012-08-17    
Medline Journal Info:
Nlm Unique ID:  0165543     Medline TA:  J Gen Appl Microbiol     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  43-51     Citation Subset:  IM    
Affiliation:
Department of Environmental Science and Engineering, Graduate School of Science and Engineering, Yamaguchi University, Yamaguchi 753-8512, Japan. miyakawa@yamaguchi-u.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Carbocyanines / metabolism
Cell Nucleus / genetics,  metabolism
Cell Nucleus Shape*
Cell Wall / metabolism
Culture Media / metabolism
Genome, Mitochondrial*
Indoles / metabolism
Meiosis*
Microscopy, Fluorescence
Microscopy, Phase-Contrast
Mitochondria / genetics*,  metabolism,  physiology
Saccharomycetales / genetics*,  metabolism,  physiology
Spores, Fungal / genetics,  metabolism,  physiology*
Staining and Labeling
Chemical
Reg. No./Substance:
0/Carbocyanines; 0/Culture Media; 0/Indoles; 47165-04-8/DAPI; 54501-79-0/3,3'-dihexyl-2,2'-oxacarbocyanine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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