| Morphology, growth, and gene expression in five newly isolated murine hepatocellular tumor cell lines. | |
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MedLine Citation:
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PMID: 7520356 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Five murine hepatocellular tumor cell lines (HepM-1-5) were isolated and grown in a synthetic medium added with hormones, growth factors and/or serum. The morphology of these lines ranged from a nearly homogeneous epithelial-like shape (HepM-2) to a stromal appearance (HepM-1). The remaining lines displayed a mixed morphology. For their proliferation all of the cell lines retained a clear dependence on the extracellular calcium level and hormonal and/or serum growth factors and, rather homogeneously, they did not express the albumin, alpha-fetoprotein (with the exception of HepM-2 cells), tyrosine aminotransferase, and ornithine transcarbamylase genes, whereas they all exhibited discrete levels of the ornithine aminotransferase mRNA. Only HepM-3 and HepM-5 lines expressed the procollagen type I gene. |
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Authors:
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T A Dragani; M Ribecco; G Manenti; M A Pierotti; L Testolin; C Guerriero; L Menapace; U Armato |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cancer letters Volume: 83 ISSN: 0304-3835 ISO Abbreviation: Cancer Lett. Publication Date: 1994 Aug |
Date Detail:
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Created Date: 1994-09-22 Completed Date: 1994-09-22 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7600053 Medline TA: Cancer Lett Country: IRELAND |
Other Details:
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Languages: eng Pagination: 269-76 Citation Subset: IM |
Affiliation:
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Division of Experimental Oncology A, Istituto Nazionale Tumori, Milan, Italy. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Division Collagen / genetics Gene Expression Regulation, Neoplastic* Liver / physiology Liver Neoplasms, Experimental / physiopathology* Male Mice Mice, Inbred A Ornithine Carbamoyltransferase / genetics Ornithine-Oxo-Acid Transaminase / genetics RNA, Messenger / genetics* RNA, Neoplasm / genetics* Serum Albumin / genetics Tumor Cells, Cultured Tyrosine Transaminase / genetics alpha-Fetoproteins / genetics |
| Chemical | |
Reg. No./Substance:
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0/RNA, Messenger; 0/RNA, Neoplasm; 0/Serum Albumin; 0/alpha-Fetoproteins; 9007-34-5/Collagen; EC 2.1.3.3/Ornithine Carbamoyltransferase; EC 2.6.1.13/Ornithine-Oxo-Acid Transaminase; EC 2.6.1.5/Tyrosine Transaminase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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