Document Detail

Morphology and density of initial lymphatics in human myocardium determined by immunohistochemistry.
MedLine Citation:
PMID:  14571339     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Myocardial edema is a common finding in congestive heart failure, transplant rejection, and cardiomyopathy. Although pathological alterations in the lymphatic morphology are part of the underlying disease, knowledge on these changes is limited. However, lymphatic morphology may be investigated by immunohistochemical staining for fms-like tyrosine 4 kinase (flt-4), which is specific for lymphatic endothelium in adult tissue. METHODS: We used myocardial tissue of ventricular out flow tract taken from five human semilunar valves harvested as allografts but unsuitable for implantation for analysis, performing immunohistochemical staining for flt-4 with a commercially available antibody. Lymphatic morphometry was completed according Gundersen method. RESULTS: Immunohistochemical staining for flt-4 resulted in successful labeling of lymph capillaries in adult human myocardium. Lymph capillary density was calculated as 50.7 +/- 12.5 per mm2 and average diameter was 3.7 +/- 0.7 microm. Conclusions. Lymph capillary morphology in human myocardium may be successfully determined by immunohistochemical staining for flt-4. Tissue samples as small as myocardial biopsies may be used for analysis. Using this method, morphological changes in myocardial lymphatics may be investigated in various cardiovascular diseases.
H J Geissler; W Bloch; S Förster; U Mehlhorn; A Krahwinkel; A Kroener; K Addicks; E R deVivie
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Thoracic and cardiovascular surgeon     Volume:  51     ISSN:  0171-6425     ISO Abbreviation:  Thorac Cardiovasc Surg     Publication Date:  2003 Oct 
Date Detail:
Created Date:  2003-10-22     Completed Date:  2004-03-18     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  7903387     Medline TA:  Thorac Cardiovasc Surg     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  244-8     Citation Subset:  IM    
Department of Cardiothoracic Surgery, University of Cologne, Germany.
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MeSH Terms
Immunohistochemistry / methods
Lymphatic System / anatomy & histology*
Myocardium / cytology*
Vascular Endothelial Growth Factor Receptor-3 / biosynthesis
Reg. No./Substance:
EC Endothelial Growth Factor Receptor-3

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