Document Detail

Morphology of cells malignantly transformed by alpha particle irradiation.
MedLine Citation:
PMID:  7347429     Owner:  NLM     Status:  MEDLINE    
The idea that one alpha particle (with LET approximately 100keV/micrometers) traversing a cell nucleus would kill a cell has been a concept which has been traditionally accepted by most radiation biologists. It was, therefore, difficult to see how alpha radiation could act directly on nuclear DNA to cause cancer. In experiments where mouse embryo cells (C3H 10T1/2) were irradiated with a parallel beam of 5.6 MeV alpha particles from a Tandem Van de Graaff machine, we made the surprising discovery that about 10 to 20 alpha particles through each nucleus were required to kill 2/3 of the cells (Lloyd 1979a). Earlier workers were misled because they failed to make measurements of nuclear areas of the cells as they were irradiated. We have used scanning electron microscopy, both to observe the cells at the time of irradiation and to document the change in unirradiated cellular dimensions as the cells become flattened on conventional tissue culture plates. The present paper also describes the morphological changes observed by SEM between cells which were subsequently transformed to become malignant by alpha irradiation and untransformed control cells.
E L Lloyd; C B Henning
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Scanning electron microscopy     Volume:  4     ISSN:  0586-5581     ISO Abbreviation:  Scan Electron Microsc     Publication Date:  1981  
Date Detail:
Created Date:  1982-12-02     Completed Date:  1982-12-02     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0371617     Medline TA:  Scan Electron Microsc     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  87-92     Citation Subset:  IM    
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MeSH Terms
Alpha Particles*
Cell Transformation, Neoplastic*
Cells, Cultured
Fibroblasts / radiation effects,  ultrastructure
Mice, Inbred C3H
Microscopy, Electron / methods
Microscopy, Electron, Scanning / methods

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