Document Detail


Morphological and physiological responses of Campylobacter jejuni to stress.
MedLine Citation:
PMID:  17133821     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Under conditions of stress, cells of Campylobacter assume a coccoid shape that may be an evolutionary strategy evolved by the organism to enable survival between hosts. However, the physiology of Campylobacter as it devolves from spiral to coccoid-shaped morphology is poorly understood. In this study, conditions influencing the survival of Campylobacter jejuni ATCC 35921 in broth were determined. Cells in late log phase were resuspended in broth at 4 or 60 degrees C. The culturability of these cold- or heat-stressed cell suspensions was determined by spread plate counts and the activity of cells by the direct viable count technique and 5-cyano-2,3-ditolyltetrazolium chloride staining. C. jejuni changed form completely from culturable to viable but nonculturable cells (VBNC) within 25 days at 4 degrees C, and 15 min at 60 degrees C. Light microscopy of C. jejuni VBNC cells showed that the spiral-shaped cells became coccoid, and transmission electron microscopy of C. jejuni VBNC cells showed that the outer membrane was lost in aging cell suspensions. Furthermore, a limited proteomic study was carried out to compare C. jejuni proteins that exhibited increased or decreased synthesis on exposure to 60 degrees C.
Authors:
Pussadee Tangwatcharin; Suganya Chanthachum; Prapaporn Khopaibool; Mansel W Griffiths
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of food protection     Volume:  69     ISSN:  0362-028X     ISO Abbreviation:  J. Food Prot.     Publication Date:  2006 Nov 
Date Detail:
Created Date:  2006-11-30     Completed Date:  2006-12-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7703944     Medline TA:  J Food Prot     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2747-53     Citation Subset:  IM    
Affiliation:
Department of Food Science and Technology, Prince of Songkla University, Songkhla 90112, Thailand.
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MeSH Terms
Descriptor/Qualifier:
Adaptation, Physiological*
Campylobacter jejuni / cytology,  growth & development,  physiology*,  ultrastructure
Colony Count, Microbial
Electrophoresis, Gel, Two-Dimensional
Fluorescence
Food Microbiology
Microbial Viability
Microscopy, Electron, Transmission
Temperature*
Time Factors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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