Document Detail


Morphological and physiological changes of Staphylococcus aureus exposed to hypochlorous acid.
MedLine Citation:
PMID:  14962048     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIMS: To characterize hypochlorous acid (HOCl) stress resistance of Staphylococcus aureus and to assess physiological state and changes in cell morphology. METHODS AND RESULTS: Clinical wild-type strain of S. aureus was used in the stress with HOCl at concentrations ranging from 0 to 4 mg l(-1). Concentrations below 1.5 mg l(-1) caused no significant drop in viability. During 2 h of HOCl stress at 2 mg l(-1), there was appearance of minicells capable of passing through the 0.45 microm pores of filtration membranes. Intracellular proteins increased gradually to reach a level of 51% of dry weight and an enhanced synthesis of at least two proteins of 23 and 220 kDa was concluded. CONCLUSIONS, SIGNIFICANCE AND IMPACT OF THE STUDY: Staphylococcus aureus can undergo morphological and physiological changes during 2 h of exposure to 2 mg l(-1) of HOCl, which represents an adaptative response towards the hypochlorous acid stress. This evolution limits the use of 0.45 microm pores size membrane filters for research on S. aureus in waters and the clinical environment.
Authors:
N Abid; S Maalej; S Rouis
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Letters in applied microbiology     Volume:  38     ISSN:  0266-8254     ISO Abbreviation:  Lett. Appl. Microbiol.     Publication Date:  2004  
Date Detail:
Created Date:  2004-02-13     Completed Date:  2004-04-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8510094     Medline TA:  Lett Appl Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  245-50     Citation Subset:  IM    
Affiliation:
Laboratoire de Microbiologie, Faculté des Sciences de Sfax, Sfax, Tunisia.
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MeSH Terms
Descriptor/Qualifier:
Bacterial Proteins / metabolism
Colony Count, Microbial
Filtration
Hypochlorous Acid / pharmacology*
Oxidants / pharmacology
Phosphoric Monoester Hydrolases / metabolism
Staphylococcus aureus / cytology,  drug effects*,  metabolism
Time Factors
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Oxidants; 7790-92-3/Hypochlorous Acid; EC 3.1.3.-/Phosphoric Monoester Hydrolases

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