Document Detail


Morphological, morphometrical and ultrastructural characterization of Phrynops geoffroanus' (Testudines: Chelidae) blood cells, in different environments.
MedLine Citation:
PMID:  20650642     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The aim of this study was to evaluate the formed elements in the periferical blood of two amostral groups of Phrynops geoffroanus: one from an urban environment under domestic sewage dumping, and another from a non-contaminated environment. Blood samples of 36 animals (females and males) were collected through cardiocentesis. Sixteen specimens were from the urban environment, and 20 were from a control environment. Samples of blood tissue were used for light microscopy analysis, and also for morphometric analysis of red blood cells. For the ultrastructural analysis, blood samples of 2 animals were used. The formed elements found, using morphological and ultrastructural analysis were: nucleated red blood cells; thrombocytes; neutrophils, lymphocytes, monocytes, basophils; eosinophils; heterophils, and azurophils. The morphometric analysis of all red blood cells parameters examinated in females showed a statistically significant difference, but in males just the nuclear area showed difference between the specimens of the two environments. The elements identified by light microscopy were elucidated by electron transmission microscopy. This P. geoffroanus study is the first one that makes a correlation between these environments and the description of turtle's blood cells, thus contributing to the identification of the hematological characteristics of this group.
Authors:
Carlos Eduardo Saranz Zago; Tiago Lucena da Silva; Maria Isabel Afonso da Silva; Larissa Paola Rodrigues Venancio; Priscila Pasqüetto Mendonça; Luiz Roberto Falleiros Junior; Luiz Dino Vizotto; Sebastião Roberto Taboga; Claudia Regina Bonini-Domingos; Maria Tercília Vilela de Azeredo-Oliveira; Classius de Oliveira
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-06-25
Journal Detail:
Title:  Micron (Oxford, England : 1993)     Volume:  41     ISSN:  1878-4291     ISO Abbreviation:  Micron     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-09-13     Completed Date:  2010-12-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9312850     Medline TA:  Micron     Country:  England    
Other Details:
Languages:  eng     Pagination:  1005-10     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier Ltd. All rights reserved.
Affiliation:
Departamento de Biologia, Centro de Estudos de Quelônios, UNESP-IBILCE-São José do Rio Preto, SP, Brazil.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biometry
Blood Cells / cytology*,  ultrastructure*
Chordata / physiology*
Environmental Exposure*
Female
Male
Microscopy
Water Pollution

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