| Morphological and histochemical pattern of response in rat testes after administration of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). | |
| | |
MedLine Citation:
|
PMID: 1666529 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Testes of rats, which had been injected with a single dose of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) (0.3 micrograms/kg-25 micrograms/kg body weight [BW]), were studied after 7 days using morphological and histochemical means. Light and electron microscopic examination revealed that TCDD affected testicular morphology in a dose-dependent manner. TCDD led to decreased intercellular contact, indicated by wide intercellular spaces between Sertoli cells between and Sertoli cells and neighbouring germ cells. Morphological alaterations in rat testes after TCDD administration included the sloughing off of premature spermatids into the tubular lumen and numerical increase of necrotic germ cells, in particular pachytene spermatocytes. Compared with control animals, Sertoli cells of treated rats exhibited an increased amount of lipid droplets and phagolysosomes. Vacuolization of the cytoplasm and fragmentation of the Sertoli cells occurred frequently. Examination of the different spermatogenic stages revealed that no stage was specifically susceptible to TCDD. In Leydig cells a decrease in enzyme activity of 3 beta- and 17 beta-hydroxysteroid dehydrogenases became evident by histochemical investigation. This effect on steroidogenesis was already found at a dose of 1 microgram/kg BW TCDD, whereas morphological effects were seen in the germinal epithelium for the first time at 3 micrograms/kg BW. |
| | |
Authors:
|
G M Rune; P de Souza; R Krowke; H J Merker; D Neubert |
Related Documents
:
|
18391539 - A cellular study of human testis development. 16770349 - Are the relationships between early activation of lymphocytes and cortisol or testoster... 15964619 - Characteristics of rat round spermatids differentiated from spermatogonial cells during... 2560809 - Human chorionic gonadotropin regulates expression of the proenkephalin gene in adult ra... 10906039 - Effects of androgen on androgen receptor expression in rat testicular and epididymal ce... 16728019 - Increased germ cell degeneration and reduced germ cell:sertoli cell ratio in stallions ... 9889049 - Antisense blockade of p21/waf1 decreases radiation-induced g2 arrest in esophageal squa... 19127649 - Calorimetric evaluations of bacillus subtilis vegetative and spore cells colonial growt... 2561999 - Effector-target co-aggregation as a crucial step in the neutrophil-mediated tumour cell... |
Publication Detail:
|
Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Histology and histopathology Volume: 6 ISSN: 0213-3911 ISO Abbreviation: Histol. Histopathol. Publication Date: 1991 Oct |
Date Detail:
|
Created Date: 1992-05-06 Completed Date: 1992-05-06 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 8609357 Medline TA: Histol Histopathol Country: SPAIN |
Other Details:
|
Languages: eng Pagination: 459-67 Citation Subset: IM |
Affiliation:
|
Department of Anatomy, Free University of Berlin, FRG. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
17-Hydroxysteroid Dehydrogenases
/
analysis* 3-Hydroxysteroid Dehydrogenases / analysis* Animals Dose-Response Relationship, Drug Histocytochemistry Male Rats Rats, Inbred Strains Sertoli Cells / drug effects, ultrastructure Spermatozoa / drug effects, ultrastructure Testis / drug effects*, enzymology, ultrastructure Tetrachlorodibenzodioxin / pharmacology* |
| Chemical | |
Reg. No./Substance:
|
1746-01-6/Tetrachlorodibenzodioxin; EC 1.1.-/17-Hydroxysteroid Dehydrogenases; EC 1.1.-/3-Hydroxysteroid Dehydrogenases; EC 1.1.1.51/3 (or 17)-beta-hydroxysteroid dehydrogenase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Conformational changes in proteins probed by hydrogen-exchange electrospray-ionization mass spectrom...
Next Document: Functional antagonism in rabbit pulmonary veins contracted by endothelin.