Document Detail


Morphological heterogeneity of Paracoccidioides brasiliensis: relevance of the Rho-like GTPase PbCDC42.
MedLine Citation:
PMID:  22493946     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Paracoccidioides brasiliensis budding pattern and polymorphic growth were previously shown to be closely linked to the expression of PbCDC42 and to influence the pathogenesis of the fungus. In this work we conducted a detailed morphogenetic evaluation of the yeast-forms of 11 different clinical and environmental P. brasiliensis isolates comprising four phylogenetic lineages (S1, PS2, PS3 and Pb01-like), as well as a PbCDC42 knock-down strain. High variations in the shape and size of mother and bud cells of each isolate were observed but we did not find a characteristic morphologic profile for any of the phylogenetic groups. In all isolates studied, the bud size and shape were demonstrated to be highly dependent on the mother cell. Importantly, we found strong correlations between PbCDC42 expression and both the shape of mother and bud cells and the size of the buds in all isolates and the knock-down strain. Our results suggested that PbCDC42 expression can explain approximately 80% of mother and bud cell shape and 19% of bud cell size. This data support PbCDC42 expression level as being a relevant predictor of P. brasiliensis morphology. Altogether, these findings quantitatively describe the polymorphic nature of the P. brasiliensis yeast form and provide additional support for the key role of PbCDC42 expression on yeast cell morphology.
Authors:
João F Menino; Nuno S Osório; Mark H J Sturme; Diana Barros; Ana G Gomes-Alves; Agostinho J Almeida; Paula Ludovico; Patricio Costa; Gustavo H Goldman; Fernando Rodrigues
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-04-12
Journal Detail:
Title:  Medical mycology     Volume:  50     ISSN:  1460-2709     ISO Abbreviation:  Med. Mycol.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-09-18     Completed Date:  2013-01-28     Revised Date:  2013-12-13    
Medline Journal Info:
Nlm Unique ID:  9815835     Medline TA:  Med Mycol     Country:  England    
Other Details:
Languages:  eng     Pagination:  768-74     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Environmental Microbiology
Gene Knockdown Techniques
Humans
Microscopy
Paracoccidioides / cytology*,  enzymology*,  isolation & purification
Paracoccidioidomycosis / microbiology
Polymorphism, Genetic*
cdc42 GTP-Binding Protein, Saccharomyces cerevisiae / metabolism*
Chemical
Reg. No./Substance:
EC 3.6.5.2/cdc42 GTP-Binding Protein, Saccharomyces cerevisiae

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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