Document Detail

Morphological and cytogenetic studies of dominant lethality induced by mitomycin C and cyclophosphamide in female germ cells. The use of Robertsonian translocations as a 'marker system' to identify the zygote pronuclei.
MedLine Citation:
PMID:  6433189     Owner:  NLM     Status:  MEDLINE    
Dominant lethal tests were performed on female mice injected intraperitoneally with cyclophosphamide (200 mg/kg) or with mitomycin C (0.2 or 5 mg/kg) at the preovulatory stage of oogenesis. Complementary experiments were undertaken to clarify the results obtained. Embryo culture showed that sterility found after treatment with cyclophosphamide or with the high dose of mitomycin C was the reflection of true dominant lethal effects. Mortality after cyclophosphamide treatment occurred predominantly at the 2- and 3-cell stages, while it was reported in all preimplantation stages after treatment with the high dose of mitomycin C. Embryos treated with the low dose of mitomycin C developed normally to the blastocyst stage, confirming the absence of preimplantation effects found with this dose in the dominant lethal test. Cytogenetic analysis of female pronuclei at the first cleavage division were performed after mating treated females with males homozygous for one Robertsonian translocation. This method allowed one to distinguish easily the female pronuclei from the male ones, which exhibited one translocated 'marker' chromosome. After treatment with cyclophosphamide, most female pronuclei showed multiple chromatid exchanges or shattering of the entire genome. After treatment with the high dose of mitomycin C, various types of premature chromosome condensation were found, and they were often accompanied by important interchromosome associations. After treatment with the low dose of mitomycin C, no structural chromosome aberrations were found, and the number of numerical anomalies was not significantly different from that found in control embryos. These last results suggest that the increase in rate of postimplantation loss obtained in the dominant lethal test with the low dose of mitomycin C was not due to clastogenic effects of this compound in the female germ cells, but rather to indirect effects on the maternal organism.
P Jacquet; P Pire
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Mutation research     Volume:  128     ISSN:  0027-5107     ISO Abbreviation:  Mutat. Res.     Publication Date:  1984 Sep 
Date Detail:
Created Date:  1984-10-24     Completed Date:  1984-10-24     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0400763     Medline TA:  Mutat Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  181-94     Citation Subset:  IM    
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MeSH Terms
Antibiotics, Antineoplastic / toxicity*
Blastocyst / drug effects
Chromosome Aberrations
Cyclophosphamide / toxicity*
Genes, Dominant / drug effects*
Genes, Lethal / drug effects*
Mice, Inbred BALB C
Mitomycins / toxicity*
Morula / drug effects
Mutagenicity Tests
Oogenesis / drug effects*
Ovum / drug effects*,  physiology
Ploidies / drug effects
Translocation, Genetic / drug effects*
Reg. No./Substance:
0/Antibiotics, Antineoplastic; 0/Mitomycins; 0/Mutagens; 50-07-7/Mitomycin; 50-18-0/Cyclophosphamide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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