Document Detail


Morphological and cytochemical characteristics of purified murine splenic dendritic cells.
MedLine Citation:
PMID:  17301370     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Dendritic cells function as the main cellular population responsible for professional antigen presentation and hence for induction of primary immune responses. Although they are present in virtually every tissue, nevertheless their number is usually so low that it makes their isolation for studies very difficult. In this study, we purified dendritic cells from mouse spleen by a three-step enrichment method and evaluated morphological and cytochemical characteristics of isolated cells. We showed that isolated dendritic cells from mouse spleen had all lobulated nuclei with multiple cytoplasmic projections and their morphological features changed after an overnight incubation. It was also shown that typical dendritic cells lacked both Myeloperoxidase (MPO) and Non Specific Esterase (NSE) activity. In conclusion, for reaching a reasonable purity in isolation of dendritic cells from lymphoid tissues, many enrichment steps should be taken, and for determining the purity of isolated cells, we recommend that a combination of morphological and cytochemical studies be used.
Authors:
A H Zarnani; S M Moazzeni; F Shokri; Modzdeh Salehnia; A A Bayat; S A R Mahmoodi; Pouneh Dokouhaki; M Jeddi Tehrani
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Iranian journal of allergy, asthma, and immunology     Volume:  2     ISSN:  1735-1502     ISO Abbreviation:  Iran J Allergy Asthma Immunol     Publication Date:  2003 Sep 
Date Detail:
Created Date:  2007-02-15     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101146178     Medline TA:  Iran J Allergy Asthma Immunol     Country:  Iran    
Other Details:
Languages:  eng     Pagination:  139-44     Citation Subset:  -    
Affiliation:
Department of Immunology, Faculty of Medical Science, Tarbiat Modarres University, Tehran, Iran. moazzeni@dr.com.
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