Document Detail


Morphological characterization of the ovary under normal cycling in rats and its viewpoints of ovarian toxicity detection.
MedLine Citation:
PMID:  19265285     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Identification of ovarian toxicity is very important for safety assessment of drugs and other environmental chemicals. The detection of interference with ovarian function is very hard without a thorough understanding of the normal ovarian morphology based on reproductive physiology. The focus of the present study was therefore a practical analysis in each stage of the estrous cycles using ovaries obtained from 143 rats demonstrating normal cycling. Transversely dissected maximum areas in the ovaries were examined microscopically for the two major features, follicles and corpora lutea (CL). Classification of growing follicles was in reference to Pedersen and Peters (1968), and functionally divided into follicular stimulating hormone (FSH)-independent and dependent categories. The former, small and medium-sized follicles, respectively primordial/primary and preantral follicles, could be readily detected by immunohistochemical staining for proliferating cell nuclear antigen (PCNA). The large antral and Graafian follicles and large sized atretic follicles showed sequential changes depending on the estrous cycle stage. CL could be divided into currently and previously formed examples. Currently formed CL underwent remarkable changes in their appearance with the cycle, reflecting ovulation and progesterone production. Thus morphological analysis that is synchronized the large antral follicle changes with recently formed CL ones allows the ovary to be classified into the each estrous cycle stage. Morphological deviation from any synchronized combination provides a first pointer of ovarian toxicity. PCNA immunohistochemical staining is also useful to detect small follicles.
Authors:
Midori Yoshida; Atsushi Sanbuissyo; Shigeru Hisada; Michihito Takahashi; Yasuo Ohno; Akiyoshi Nishikawa
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of toxicological sciences     Volume:  34 Suppl 1     ISSN:  1880-3989     ISO Abbreviation:  J Toxicol Sci     Publication Date:  2009  
Date Detail:
Created Date:  2009-03-06     Completed Date:  2009-06-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7805798     Medline TA:  J Toxicol Sci     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  SP189-97     Citation Subset:  IM    
Affiliation:
Division of Pathology, Biology Safety Research Center, National Institute of Health Sciences, Tokyo, Japan. midoriy@nihs.go.jp
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MeSH Terms
Descriptor/Qualifier:
Animals
Corpus Luteum / anatomy & histology,  physiology
Estrous Cycle / physiology*
Female
Ovarian Follicle / anatomy & histology,  physiology
Ovary / anatomy & histology*,  physiology*
Rats
Rats, Inbred Strains
Toxicity Tests / methods*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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