Document Detail

Morphological changes of myelin sheaths in rats intracranially injected with apotransferrin.
MedLine Citation:
PMID:  12587668     Owner:  NLM     Status:  MEDLINE    
Previous findings from our laboratories indicate that the intracranial injection of apotransferrin (aTf) in neonatal rats produces an accelerated oligodendrocyte maturation and an enhanced production and deposition of myelin membranes in the brain. To evaluate the anatomical distribution and the morphological characteristics of the myelin in these rats, we analyzed the optic nerves, cerebellum, and selected areas of brain sections from aTf-treated and control rats by both light and electron microscopy. Microscopic identification of myelin using a specific staining procedure, showed that in aTf-injected rats, in coincidence with previous biochemical studies, there was an increased deposition of myelin in selected areas of the nervous system. Qualitative and quantitative analysis of electron micrographs from areas showing increased myelinaton, such as the optic nerves and the corpus callosum, showed that among other changes, the intracranial treatment with aTf produces ultrastructural evidences of myelin decompaction, consisting of an enlargement in the distance between adjacent major dense lines, a decreased density of the intraperiod line, and an increased electron density of the major dense line, accompanied by a significant increase in its width. The intracranial administration of aTf induces an increased deposition of myelin by oligodeudroglial cells (OLGc), and these myelin membranes, in spite of the changes in composition and in morphology, appear to function normally. Apotransferrin can be considered as a differentiation factor that could be used to stimulate remyelination in cases in which myelin has been destroyed by various pathological processes.
Cecilia B Marta; Pablo Paez; Margarita Lopez; Amanda Pellegrino de Iraldi; Eduardo F Soto; Juana M Pasquini
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neurochemical research     Volume:  28     ISSN:  0364-3190     ISO Abbreviation:  Neurochem. Res.     Publication Date:  2003 Jan 
Date Detail:
Created Date:  2003-02-17     Completed Date:  2003-04-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7613461     Medline TA:  Neurochem Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  101-10     Citation Subset:  IM    
Departamento de Quimica Biológica and Instituto de Química y Fisicoquímica Biológicas (IQUIFIB), UBA-CONICET, Facultad de Farmacia y Bioquimica, Universidad de Buenos Aires, Buenos Aires, Argentina.
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MeSH Terms
Apoproteins / administration & dosage*
Microscopy, Electron
Myelin Sheath / pathology*,  ultrastructure
Rats, Wistar
Transferrin / administration & dosage*
Reg. No./Substance:
0/Apoproteins; 0/apotransferrin; 11096-37-0/Transferrin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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