Document Detail


Morphological changes of Vibrio parahaemolyticus under cold and starvation stresses.
MedLine Citation:
PMID:  19101053     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study addresses morphological changes of Vibrio parahaemolyticus cells during the initial period of stress. Drastic changes occurred in cells under starvation with/without concomitant cold treatment for one to a few days. Under carbon starvation, rod-shaped cells became coccoid by reductive division, which was influenced by glucose/amino acid in the culture medium. Under concomitant cold and carbon starvation, cells entered the viable but nonculturable state and their shape changed from rod-like to coccoid with waning-shape and various aberrantly intermediates, such as club-shaped, budding, club-shaped with budding, dumbbell-shaped and rabbit-ear-like-shaped. Electron microscopy revealed characteristic features in the cells that were concomitantly stressed with cold and starved, such as densely stained peripheral part and lightly stained central part of cytoplasm, and a thick peptidoglycan cell wall in the temperature-upshifted cells. The expression of cytoskeleton genes, mreB, minE and ftsZ, was determined in a real-time quantitative reverse transcription-polymerase chain reaction, and results revealed a substantial decline in expression in cells under starvation (to 0.25 to 0.64 times that of the control) in the first 2 to 3 h, and a rapid upregulated expression in recovering cells. Alteration of the morphology of cells under such stresses may be associated with the expression of these cytoskeleton genes. This work demonstrates the uniqueness of concomitant cold and starvation treatment in V. parahaemolyticus. The details of the morphological changes may facilitate understanding of stress adaptation in bacteria.
Authors:
Shau-Yan Chen; Wann-Neng Jane; Yi-Shin Chen; Hin-Chung Wong
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-11-17
Journal Detail:
Title:  International journal of food microbiology     Volume:  129     ISSN:  1879-3460     ISO Abbreviation:  Int. J. Food Microbiol.     Publication Date:  2009 Feb 
Date Detail:
Created Date:  2009-02-05     Completed Date:  2009-05-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8412849     Medline TA:  Int J Food Microbiol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  157-65     Citation Subset:  IM    
Affiliation:
Department of Microbiology, Soochow University, Taipei, Taiwan 111, Republic of China.
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MeSH Terms
Descriptor/Qualifier:
Adaptation, Physiological*
Cold Temperature*
Colony Count, Microbial
Consumer Product Safety
Cytoskeleton / genetics*
Food Microbiology
Gene Expression
Gene Expression Regulation, Bacterial*
Vibrio parahaemolyticus / cytology,  physiology*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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