Document Detail


Morphological and biochemical alterations of oomycete fish pathogen Saprolegnia parasitica as affected by salinity, ascorbic acid and their synergistic action.
MedLine Citation:
PMID:  15770449     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Vegetative growth of Saprolegnia parasitica decreased by increasing the concentration of NaCl and ascorbic acid. Under these conditions, the morphological features of the vegetative hyphae were distinguishable from those used as controls. NaCl and ascorbic acid in combination improved the tolerance of S. parasitica to high levels of salinity. Sporangial formation, release and proliferation were very sensitive to even lower levels of salinity. For instance, at 0.03 M NaCl sporangia formation was rarely observed. Ascorbic acid alone had a little effect on sporangial formation and release, but when combine with NaCl the developmental processes were improved. Reduction of numbers and plasmolysis of oogonia were found at various NaCl concentrations, whereas ascorbic acid stimulated the formation of these reproductive organs at low concentrations. The synergistic effect of NaCl and ascorbic acid improved and overcomed the symptoms of oogonial plasmolysis. Protease activity of S. parasitica was significantly reduced at all NaCl concentrations, whilst ascorbic acid significantly increased and inhibited it at low concentrations and at moderate and high concentrations, respectively. The combination of these compounds reduced protease activity at all tested concentrations with significant difference at the highest concentration. The total free amino-acids content of S. parasitica mycelia was significantly reduced at all the NaCl concentrations, whereas ascorbic acid significantly increased it at low but inhibited it at higher concentrations. The combination of NaCl and ascorbic acid significantly increased the accumulation of free amino-acids at low and moderate concentrations, but decreased them at high concentrations. Total protein content was reduced at all tested concentrations of NaCl and ascorbic acid had also similar effect. However, the combined effect of NaCl and ascorbic acid significantly enhanced and reduced total protein content at low and high concentrations, respectively. Treatments with NaCl induced proline accumulation in S. parasitica, which paralleled the salt concentration.
Authors:
Esam H Ali
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Mycopathologia     Volume:  159     ISSN:  0301-486X     ISO Abbreviation:  Mycopathologia     Publication Date:  2005 Feb 
Date Detail:
Created Date:  2005-03-16     Completed Date:  2005-05-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7505689     Medline TA:  Mycopathologia     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  231-43     Citation Subset:  IM    
Affiliation:
Faculty of Science, Department of Botany, Assiut University, Assiut, Egypt. ibraheem55@yahoo.com
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MeSH Terms
Descriptor/Qualifier:
Animals
Ascorbic Acid / pharmacology*
Cellulase / metabolism
Peptide Hydrolases / metabolism
Proline / metabolism
Saprolegnia / drug effects*,  enzymology,  growth & development*,  metabolism
Sodium Chloride / pharmacology*
Water Microbiology
Chemical
Reg. No./Substance:
147-85-3/Proline; 50-81-7/Ascorbic Acid; 7647-14-5/Sodium Chloride; EC 3.2.1.4/Cellulase; EC 3.4.-/Peptide Hydrolases

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