Document Detail

Morphological alteration of X-ray induced partially transformed human cells by transfection with a small c-myc DNA sequence.
MedLine Citation:
PMID:  10860847     Owner:  NLM     Status:  MEDLINE    
During attempts to transform a normal human fibroblast strain (GM730) by X-irradiation, we obtained a partially transformed cell strain (GM730pt) which demonstrates several aspects of the transformed phenotype including morphological changes, increased saturation density, growth in soft agar, and focus formation in long-term cultures. When GM730pt cells were transfected with the feline c-myc gene, morphology of the cells changed dramatically following seven days of expression. Transfection of other plasmid DNAs or oncogenes such as pUC8, pSV2neo, src, sis, and H-ras had little or no effects on the phenotype of GM730pt cells. On the other hand, a gel purified, small fragment of c-myc DNA had a complete cell alteration activity. Furthermore, Bal 31 deletion and M13 sequencing experiments showed that the alteration seen in GM730pt cells is delimited to a 24 nucleotide stretch (active myc element) from the second intron of the feline c-myc gene that contains a T-rich sequence.
Y Kano; T Nohno; R Takahashi; F Hiragami; K Kawamura; K Strebhardt; M Namba; T Sugiyama; J B Little
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  272     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2000 Jun 
Date Detail:
Created Date:  2000-07-26     Completed Date:  2000-07-26     Revised Date:  2006-05-01    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  887-94     Citation Subset:  IM    
Copyright Information:
Copyright 2000 Academic Press.
Department of Health Science, Kibi International University, Okayama, Japan.
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MeSH Terms
Base Sequence
Blotting, Southern
Cell Count / radiation effects
Cell Division / radiation effects
Cell Line, Transformed
Cell Size / radiation effects
Cell Transformation, Neoplastic / genetics*,  pathology,  radiation effects*
Cells, Cultured
Fibroblasts / metabolism,  pathology*,  radiation effects*
Genes, myc / genetics*,  physiology*
Infant, Newborn
Introns / genetics
Middle Aged
Oncogenes / genetics,  physiology
Plasmids / genetics,  physiology
Poly T / genetics
Sequence Deletion / genetics
Time Factors
Tumor Stem Cell Assay
Reg. No./Substance:
25086-81-1/Poly T; 9002-18-0/Agar

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