Document Detail

Morphological Features of Osteoblasts Cultured on Ultraviolet-irradiated Titanium Plates.
MedLine Citation:
PMID:  21709009     Owner:  NLM     Status:  In-Data-Review    
BACKGROUND: Although we have recently established optimal experimental conditions of ultraviolet (UV)-irradiation for titanium plates (e.g. wavelength and exposure time) which enhanced osteoblast adhesion to the plates, the effects of UV-irradiation on cell structure are still unclear.
MATERIALS AND METHODS: Digital stereomicroscopy was used to investigate morphological alterations of non-stained viable and hematoxylin-eosin (HE)-stained cells on UV-irradiated and non-UV-irradiated titanium plates for up to 24 hours.
RESULTS: In 24 hours, significant expansion of HE-stained cells (area, perimeter and sprouting processes) was observed on UV-irradiated plates. The sprouting processes appeared within 40 minutes of inoculation under both conditions, however, significant cell area expansion, which occurred in 5 minutes, was observed only on UV-irradiated plates.
CONCLUSION: UV-enhanced cell attachment was related to morphological alteration which occurred immediately after inoculation. Digital stereomicroscopic evaluation was able to define and quantify morphological alterations of viable cells in an opaque environment.
René García-Contreras; Shuichiro Kanagawa; Yuji Beppu; Takahide Nagao; Hiroshi Sakagami; Hiroshi Nakajima; Jun Shimada; Kazunori Adachi
Related Documents :
1898329 - Phorbol-ester-mediated expression of the collagen type i pro-alpha 2 gene in mouse 3t3-...
2369749 - Identification of epidermal cell subpopulations with increased ornithine decarboxylase ...
10371299 - Establishment and characterization of normal and initiated hamster tracheal epithelial ...
1525349 - Protein kinase c activation in murine endothelioma cell lines containing middle t antig...
8401289 - Enhancement of phorbol ester induced cell aggregation after alterations in asparagine-l...
8979499 - Benzoyl peroxide modulates gene expression by epigenetic mechanism in mouse epidermal j...
20443079 - Glioma cell death: cell-cell interactions and signalling networks.
14105209 - The relations between dna, rna, and protein in normal embryonic cell nuclei and spontan...
21611769 - Lipid-soluble ginseng extract induces apoptosis and g0/g1 cell cycle arrest in nci-h460...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  In vivo (Athens, Greece)     Volume:  25     ISSN:  1791-7549     ISO Abbreviation:  In Vivo     Publication Date:    2011 Jul-Aug
Date Detail:
Created Date:  2011-06-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8806809     Medline TA:  In Vivo     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  649-55     Citation Subset:  IM    
Division of Pharmacology, Department of Diagnostic and Therapeutic Sciences, Meikai University School of Dentistry, 1-1 Keyakidai, Sakado, Saitama, 350-0283, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Effect of Melatonin on Cyclooxygenase-2 Expression and Nuclear Factor-Kappa B Activation in RAW264.7...
Next Document:  Effects of grape extracts on the in vitro activity of enzymes involved in oxidative stress regulatio...