Document Detail


Morphological Features of Osteoblasts Cultured on Ultraviolet-irradiated Titanium Plates.
MedLine Citation:
PMID:  21709009     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
BACKGROUND: Although we have recently established optimal experimental conditions of ultraviolet (UV)-irradiation for titanium plates (e.g. wavelength and exposure time) which enhanced osteoblast adhesion to the plates, the effects of UV-irradiation on cell structure are still unclear.
MATERIALS AND METHODS: Digital stereomicroscopy was used to investigate morphological alterations of non-stained viable and hematoxylin-eosin (HE)-stained cells on UV-irradiated and non-UV-irradiated titanium plates for up to 24 hours.
RESULTS: In 24 hours, significant expansion of HE-stained cells (area, perimeter and sprouting processes) was observed on UV-irradiated plates. The sprouting processes appeared within 40 minutes of inoculation under both conditions, however, significant cell area expansion, which occurred in 5 minutes, was observed only on UV-irradiated plates.
CONCLUSION: UV-enhanced cell attachment was related to morphological alteration which occurred immediately after inoculation. Digital stereomicroscopic evaluation was able to define and quantify morphological alterations of viable cells in an opaque environment.
Authors:
René García-Contreras; Shuichiro Kanagawa; Yuji Beppu; Takahide Nagao; Hiroshi Sakagami; Hiroshi Nakajima; Jun Shimada; Kazunori Adachi
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  In vivo (Athens, Greece)     Volume:  25     ISSN:  1791-7549     ISO Abbreviation:  In Vivo     Publication Date:    2011 Jul-Aug
Date Detail:
Created Date:  2011-06-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8806809     Medline TA:  In Vivo     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  649-55     Citation Subset:  IM    
Affiliation:
Division of Pharmacology, Department of Diagnostic and Therapeutic Sciences, Meikai University School of Dentistry, 1-1 Keyakidai, Sakado, Saitama, 350-0283, Japan. k-adachi@dent.meikai.ac.jp.
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