Document Detail

Morphologic changes in rat urothelial cells during carcinogenesis: II. Image cytometry.
MedLine Citation:
PMID:  6386387     Owner:  NLM     Status:  MEDLINE    
Improved early detection of neoplasia by screening of urothelial cells requires an understanding of the features distinguishing normal and neoplastic cell populations. We have begun a program of study based upon a rat model system for the controlled observation of early-stage lesions produced by the carcinogen N-butyl-N-(4-hydroxybutyl)- nitrosamine. Cells dissociated directly from normal and malignant urothelium were characterized by conventional cytopathology techniques and by quantitative microscopy (for nuclear texture and nuclear and cytoplasmic size, shape, and stain content) to derive a comprehensive picture of bladder tumor development. By following the changes that occur in the dissociated urothelial cells we have found that the nuclear area, total nuclear stain, nuclear shape, and the nuclear chromatin change significantly over a 48-wk interval as the lesions progress toward malignancy.
I T Young; M Vanderlaan; L Kromhout; R Jensen; A Grover; E King
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cytometry     Volume:  5     ISSN:  0196-4763     ISO Abbreviation:  Cytometry     Publication Date:  1984 Sep 
Date Detail:
Created Date:  1984-12-19     Completed Date:  1984-12-19     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8102328     Medline TA:  Cytometry     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  454-62     Citation Subset:  IM    
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MeSH Terms
Cell Nucleus / pathology
Chromatin / ultrastructure
Cytological Techniques*
Rats, Inbred F344
Time Factors
Urinary Bladder Neoplasms / chemically induced,  pathology*
Grant Support
Reg. No./Substance:
0/Chromatin; 3817-11-6/Butylhydroxybutylnitrosamine

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