Document Detail

Morphogenetic activity of silica and bio-silica on the expression of genes controlling biomineralization using SaOS-2 cells.
MedLine Citation:
PMID:  17957327     Owner:  NLM     Status:  MEDLINE    
In a previous study (Schröder et al., J Biomed Mater Res B Appl Biomater 75:387-392, 2005) we demonstrated that human SaOS-2 cells, when cultivated on bio-silica matrices, respond with an increased hydroxyapatite deposition. In the present contribution we investigate if silica-based components (Na-silicate, tetraethyl orthosilicate [TEOS], silica-nanoparticles) (1) change the extent of biomineralization in vitro (SaOS-2 cells) and (2) cause an alteration of the expression of the genes amelogenin, ameloblastin, and enamelin, which are characteristic for an early stage of osteogenesis. We demonstrate that the viability of SaOS-2 cells was not affected by the silica-based components. If Na-silicate or TEOS was added together with ss-glycerophosphate, an organic phosphate donor, a significant increase in biomineralization was measured. Finally, expression levels of the amelogenin, ameloblastin, and enamelin genes were determined in SaOS-2 cells during exposure to the silica-based components. After exposure for 2 days, expression levels of amelogenin and enamelin strongly increased in response to the silica-based components, while no significant change was seen for ameloblastin. In contrast, exposure of SaOS-2 cells to ss-glycerophosphate resulted in increased expression of all three genes. We conclude that the levels of the structural molecules of the enamel matrix, amelogenin and enamelin, increase in the presence of silica-based components and substantially contribute to the extent of hydroxyapatite crystallite formation. These results demonstrate that silica-based components augment hydroxyapatite deposition in vitro and suggest that enzymatically synthesized bio-silica (via silicatein) might be a promising route for tooth reconstruction in vivo.
Werner E G Müller; Alexandra Boreiko; Xiaohong Wang; Anatoli Krasko; Werner Geurtsen; Márcio Reis Custódio; Thomas Winkler; Lada Lukić-Bilela; Thorben Link; Heinz C Schröder
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-10-24
Journal Detail:
Title:  Calcified tissue international     Volume:  81     ISSN:  0171-967X     ISO Abbreviation:  Calcif. Tissue Int.     Publication Date:  2007 Nov 
Date Detail:
Created Date:  2007-11-08     Completed Date:  2008-02-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7905481     Medline TA:  Calcif Tissue Int     Country:  United States    
Other Details:
Languages:  eng     Pagination:  382-93     Citation Subset:  IM    
Institut für Physiologische Chemie, Abteilung Angewandte Molekularbiologie, Universität, Duesbergweg 6, D-55099 Mainz, Germany.
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MeSH Terms
Amelogenin / genetics
Biocompatible Materials / pharmacology*,  therapeutic use
Bone Regeneration / drug effects,  physiology
Calcification, Physiologic / drug effects*,  physiology
Cell Line, Tumor
Dental Enamel / growth & development*,  metabolism
Dental Enamel Proteins / genetics*
Gene Expression Regulation, Developmental / drug effects*,  genetics
Glycerophosphates / pharmacology
Microscopy, Electron, Scanning
RNA, Messenger / drug effects,  metabolism
Silicates / pharmacology,  therapeutic use
Silicon Dioxide / pharmacology*,  therapeutic use
Tooth / growth & development*,  metabolism
Up-Regulation / drug effects,  genetics
Reg. No./Substance:
0/AMBN protein, human; 0/Amelogenin; 0/Biocompatible Materials; 0/Dental Enamel Proteins; 0/Glycerophosphates; 0/RNA, Messenger; 0/Silicates; 0/enamel matrix proteins; 0/tuftelin; 17181-54-3/beta-glycerophosphoric acid; 7631-86-9/Silicon Dioxide

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