Document Detail

Morphine extraction in necrophagous insects remains for determining ante-mortem opiate intoxication.
MedLine Citation:
PMID:  11457620     Owner:  NLM     Status:  MEDLINE    
To study the potential use of insects remains as toxicological indicators, we measured morphine in desiccated entomological materials using a radioimmunoassay method (Coat-A-Count Serum Morphine, Dade Behring, France) following enzymatic hydrolysis.First, enzymatic cuticle extraction of morphine (pronase digestion preceded by a 2h incubation with a dithiothreitol solution) was performed on various substrates (Calliphoridae puparial cases and desiccated adults, and desiccated pupae of Dermestidae) which were then tested to identify possible interferences with the radioimmunoassay procedure. This same procedure was performed on puparial cases and desiccated adults of Lucilia sericata (Diptera, Calliphoridae) previously reared on minced meat containing various morphine concentrations. Morphine was detected only in cuticle powder from insects reared on meats containing 100 and 1000mg/kg. Higher concentrations were measured in puparial cases. Rearings on psoas from eight heroin overdose victims confirmed previous experimental results. Remains of necrophagous insects, particularly puparial cases, are often preserved for a long time, and consequently can serve as late alternative specimens for toxicological analysis when suitable tissues are not available.
B Bourel; G Tournel; V Hedouin; M Deveaux; M L Goff; D Gosset
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Forensic science international     Volume:  120     ISSN:  0379-0738     ISO Abbreviation:  Forensic Sci. Int.     Publication Date:  2001 Aug 
Date Detail:
Created Date:  2001-07-17     Completed Date:  2001-09-06     Revised Date:  2009-11-03    
Medline Journal Info:
Nlm Unique ID:  7902034     Medline TA:  Forensic Sci Int     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  127-31     Citation Subset:  IM    
Laboratoire Environnement et Santé, Faculté Libre des Sciences et Faculté Libre de Médecine, Institut Catholique de Lille, 56 rue du port, 59046 Lille Cedex, France.
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MeSH Terms
Autopsy / methods
Diptera / chemistry*
Forensic Medicine / methods*
Morphine / analysis*
Opioid-Related Disorders / diagnosis*
Overdose / diagnosis
Psoas Muscles / chemistry
Reg. No./Substance:

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