| Morin exerts neuroprotective actions in Parkinson disease models in vitro and in vivo. | |
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MedLine Citation:
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PMID: 20644549 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIM: To investigate the neuroprotective effects of morin on 1-methyl-4-phenylpyridinium ion (MPP(+))-induced apoptosis in neuronal differentiated PC12 cells as well as in a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) mouse model of Parkinson disease (PD). METHODS: PC12 cells were challenged with MPP(+) in the presence or absence of morin. Cell viability was determined using MTT assay. Cell apoptosis was measured using flow cytometry. Generation of reactive oxygen species (ROS) was assayed using fluorescence assay. In an MPTP mouse model of PD, behavioral deficits, striatal dopamine content, and number of dopaminergic neurons were measured. RESULTS: MPP(+) induced apoptosis and ROS formation in PC12 cells. Concomitant treatment with morin (5-50 mumol/L) significantly attenuated the loss of cell viability and apoptosis when compared with MPP(+) treatment alone. Morin also attenuated ROS formation induced by MPP(+). MPTP induced permanent behavioral deficits and nigrostriatal lesions in mice. When administered prior to MPTP, morin (20 to 100 mg/kg) attenuated behavioral deficits, dopaminergic neuronal death and striatal dopamine depletion in the MPTP mouse model. CONCLUSION: The findings suggest that morin has neuroprotective actions both in vitro and in vivo, and may provide a novel therapeutic agent for the treatment of PD and other neurodegenerative diseases. |
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Authors:
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Zhen-tao Zhang; Xue-bing Cao; Nian Xiong; Hong-cai Wang; Jin-sha Huang; Sheng-gang Sun; Tao Wang |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-07-19 |
Journal Detail:
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Title: Acta pharmacologica Sinica Volume: 31 ISSN: 1745-7254 ISO Abbreviation: Acta Pharmacol. Sin. Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-08-05 Completed Date: 2010-11-12 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100956087 Medline TA: Acta Pharmacol Sin Country: United States |
Other Details:
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Languages: eng Pagination: 900-6 Citation Subset: IM |
Affiliation:
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Department of Neurology, Renmin Hospital of Wuhan University, Wuhan, China. zhangzhentao104@yahoo.com.cn |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine 1-Methyl-4-phenylpyridinium Animals Antiparkinson Agents / administration & dosage, pharmacology* Apoptosis / drug effects Cell Survival / drug effects Dopamine / metabolism Dose-Response Relationship, Drug Flavonoids / administration & dosage, pharmacology* Flow Cytometry Male Mice Mice, Inbred C57BL Neuroprotective Agents / administration & dosage, pharmacology* PC12 Cells Parkinsonian Disorders / drug therapy*, physiopathology Rats Reactive Oxygen Species / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Antiparkinson Agents; 0/Flavonoids; 0/Neuroprotective Agents; 0/Reactive Oxygen Species; 28289-54-5/1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine; 480-16-0/morin; 48134-75-4/1-Methyl-4-phenylpyridinium |
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