Document Detail


More than 45% of A549 and H446 cells are cancer initiating cells: evidence from cloning and tumorigenic analyses.
MedLine Citation:
PMID:  19287999     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cancer stem cells have been isolated from human lung cancer and cancer cell lines. This study was designed to investigate the fraction of cancer stem cells in the A549 and H446 cell lines by cloning and tumorigenic analyses. We found that >45% of A549 and H446 cells formed large clones that are able to generate subclones and subsequently give rise to xenograft tumors in the BALB/C-nude mouse. Both CD133+ and CD133- cells of A549 and H446 possessed stem cell characteristics and exposure to Hoechst 33342 affected the clonogenicity and proliferation of single A549 and H446 cell. Moreover, based on cell cycle phase distribution and growth curve, most A549 and H446 cells may not be stem cells, but they acquired stem cell characteristics when they were alone. Therefore, these cells that formed large clones should be called cancer initiating cells, not stem cells. Thus, we concluded that >45% of A549 and H446 cells were cancer initiating cells, although many of them were neither CD133+ cells nor side population cells.
Authors:
Xiangjiao Meng; Xiuwen Wang; Yawei Wang
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Oncology reports     Volume:  21     ISSN:  1021-335X     ISO Abbreviation:  Oncol. Rep.     Publication Date:  2009 Apr 
Date Detail:
Created Date:  2009-03-16     Completed Date:  2009-06-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9422756     Medline TA:  Oncol Rep     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  995-1000     Citation Subset:  IM    
Affiliation:
Department of Chemotherapy, Oncology Center, Qilu Hospital, Shandong University, Jinan 250012, PR China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigens, CD / analysis
Benzimidazoles / pharmacology
Cell Cycle
Cell Line, Tumor
Cell Proliferation
Glycoproteins / analysis
Humans
Lung Neoplasms / pathology*
Mice
Mice, Inbred BALB C
Neoplastic Stem Cells / pathology*
Octamer Transcription Factor-3 / analysis
Peptides / analysis
Chemical
Reg. No./Substance:
0/AC133 antigen; 0/Antigens, CD; 0/Benzimidazoles; 0/Glycoproteins; 0/Octamer Transcription Factor-3; 0/Peptides; 23491-52-3/HOE 33342

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