| More than 45% of A549 and H446 cells are cancer initiating cells: evidence from cloning and tumorigenic analyses. | |
| | |
MedLine Citation:
|
PMID: 19287999 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Cancer stem cells have been isolated from human lung cancer and cancer cell lines. This study was designed to investigate the fraction of cancer stem cells in the A549 and H446 cell lines by cloning and tumorigenic analyses. We found that >45% of A549 and H446 cells formed large clones that are able to generate subclones and subsequently give rise to xenograft tumors in the BALB/C-nude mouse. Both CD133+ and CD133- cells of A549 and H446 possessed stem cell characteristics and exposure to Hoechst 33342 affected the clonogenicity and proliferation of single A549 and H446 cell. Moreover, based on cell cycle phase distribution and growth curve, most A549 and H446 cells may not be stem cells, but they acquired stem cell characteristics when they were alone. Therefore, these cells that formed large clones should be called cancer initiating cells, not stem cells. Thus, we concluded that >45% of A549 and H446 cells were cancer initiating cells, although many of them were neither CD133+ cells nor side population cells. |
| | |
Authors:
|
Xiangjiao Meng; Xiuwen Wang; Yawei Wang |
Related Documents
:
|
18432399 - Development of cell lines from the sheep used to construct the chori-243 ovine bac libr... 1694289 - Establishment of rat endometrial cell lines by retroviral mediated transfer of immortal... 19453019 - Rapamycin inhibits growth of cholangiocarcinoma cells. 9815919 - Human glioma cells overexpress receptors for interleukin 13 and are extremely sensitive... 1368119 - A novel vero cell line for use as a mammalian host-vector system in serum-free medium. 19394139 - Spontaneous immortalization of oligodendroglial cells derived from an sv40 t antigen-po... 2223789 - Specific electropermeabilization of leucocytes in a blood sample and application to lar... 1354509 - Suspected chemoreceptors in coelenterates and ctenophores. 14594839 - Flavobacterium johnsoniae gldh is a lipoprotein that is required for gliding motility a... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Oncology reports Volume: 21 ISSN: 1021-335X ISO Abbreviation: Oncol. Rep. Publication Date: 2009 Apr |
Date Detail:
|
Created Date: 2009-03-16 Completed Date: 2009-06-18 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9422756 Medline TA: Oncol Rep Country: Greece |
Other Details:
|
Languages: eng Pagination: 995-1000 Citation Subset: IM |
Affiliation:
|
Department of Chemotherapy, Oncology Center, Qilu Hospital, Shandong University, Jinan 250012, PR China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Antigens, CD / analysis Benzimidazoles / pharmacology Cell Cycle Cell Line, Tumor Cell Proliferation Glycoproteins / analysis Humans Lung Neoplasms / pathology* Mice Mice, Inbred BALB C Neoplastic Stem Cells / pathology* Octamer Transcription Factor-3 / analysis Peptides / analysis |
| Chemical | |
Reg. No./Substance:
|
0/AC133 antigen; 0/Antigens, CD; 0/Benzimidazoles; 0/Glycoproteins; 0/Octamer Transcription Factor-3; 0/Peptides; 23491-52-3/HOE 33342 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: A pilot study of vasculogenic mimicry immunohistochemical expression in intraocular melanoma model.
Next Document: Mutational analysis of Smad7 in human cervical cancer.