| More severe type 2 diabetes-associated ischemic stroke injury is alleviated in aldose reductase-deficient mice. | |
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MedLine Citation:
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PMID: 20143423 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Aldose reductase (AR), the first enzyme in the polyol pathway, has been implicated in a wide variety of physiological and pathological functions, such as diabetic vascular and neural complications. It is known that diabetes mellitus can exacerbate brain and retina damage after ischemic injuries. However, the underlying mechanisms are not clear. In the present study, we made use of db/db mice with an AR null mutation (AR(-/-)db/db) to understand better the role of AR in the pathogenesis of brain and retina ischemic injuries under diabetic conditions. Cerebral and retinal ischemia was induced by transient middle cerebral artery occlusion in control and diabetic mice either with or without an AR null mutation. Mice were evaluated for neurological deficits after 30 min of ischemia and 23.5 hr of reperfusion. Our results showed that the diabetic db/db mice had significantly more severe neurological deficit and larger brain infarct size than the nondiabetic mice. Compared with wild-type db/db mice, the AR(-/-)db/db mice had significantly lower neurological scores, smaller brain infarct areas, and less hemispheric brain swelling. Retinal swelling was also significantly decreased in the AR(-/-)db/db mice. Less swelling in the brain and retina of the AR(-/-)db/db mice correlated with less expression of the water channel aquaporin 4. Taken together, these data clearly show that deletion of AR leads to less severe brain and retinal ischemic injuries in the diabetic db/db mouse. The present study indicates that inhibition of AR in diabetics may protect against damage in the brain and retina following ischemic reperfusion injury. |
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Authors:
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Chung-Man Yeung; Amy C Y Lo; Alvin K H Cheung; Stephen S M Chung; David Wong; Sookja K Chung |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of neuroscience research Volume: 88 ISSN: 1097-4547 ISO Abbreviation: J. Neurosci. Res. Publication Date: 2010 Jul |
Date Detail:
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Created Date: 2010-05-17 Completed Date: 2010-09-16 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7600111 Medline TA: J Neurosci Res Country: United States |
Other Details:
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Languages: eng Pagination: 2026-34 Citation Subset: IM |
Affiliation:
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Department of Anatomy, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aldehyde Reductase
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deficiency,
genetics,
metabolism* Animals Aquaporin 4 / metabolism Blood Glucose / metabolism Brain / enzymology, metabolism, pathology Brain Ischemia / enzymology*, metabolism, pathology Diabetes Mellitus, Experimental / enzymology, metabolism, pathology Diabetes Mellitus, Type 2 / complications*, enzymology, metabolism, pathology Infarction, Middle Cerebral Artery / enzymology, metabolism, pathology Mice Mice, Inbred C57BL Mice, Knockout Mice, Transgenic Oxidative Stress / physiology Retina / enzymology, metabolism, pathology Retinal Diseases / enzymology*, metabolism, pathology Severity of Illness Index Stroke / enzymology*, metabolism, pathology |
| Chemical | |
Reg. No./Substance:
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0/Aqp4 protein, mouse; 0/Aquaporin 4; 0/Blood Glucose; EC 1.1.1.21/Aldehyde Reductase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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