Document Detail


More precise mapping of the replication origin in Escherichia coli K-12.
MedLine Citation:
PMID:  4607435     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The origin of replication in Escherichia coli K-12 was mapped by determining the rate of marker replication during a synchronous round of replication. Four isogenic strains were made lysogenic for lambdaind(-) and for phage Mu-1, with Mu-1 integrated into a different chromosomal location in each strain. Cultures were starved for amino acids to allow completion of chromosome replication cycles and then starved for thymine in the presence of amino acids, and a synchronous cycle of replication was initiated by the addition of thymine. Samples were exposed to radioactive thymidine at intervals, deoxyribonucleic acid was extracted, and the rate of marker replication was determined by deoxyribonucleic acid-deoxyribonucleic acid hybridization to filters containing Mu-1, lambda, and E. coli deoxyribonucleic acid. The results confirm that the origin of replication is near ilv. The travel times of the replication forks, calculated from the data obtained for cultures with doubling times of approximately 40 and 61 min, are 40 and 52 min, respectively.
Authors:
J Louarn; M Funderburgh; R E Bird
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of bacteriology     Volume:  120     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1974 Oct 
Date Detail:
Created Date:  1975-01-13     Completed Date:  1975-01-13     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1-5     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / metabolism
Chromosome Mapping*
Coliphages
DNA Replication*
DNA Viruses
DNA, Bacterial / biosynthesis*
Escherichia coli / metabolism*
Glucose / metabolism
Lysogeny
Nucleic Acid Hybridization
Spectrophotometry
Thymidine / metabolism
Thymine / metabolism
Tritium
Chemical
Reg. No./Substance:
0/Amino Acids; 0/DNA, Bacterial; 10028-17-8/Tritium; 50-89-5/Thymidine; 50-99-7/Glucose; 65-71-4/Thymine
Comments/Corrections

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