| More precise mapping of the replication origin in Escherichia coli K-12. | |
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MedLine Citation:
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PMID: 4607435 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The origin of replication in Escherichia coli K-12 was mapped by determining the rate of marker replication during a synchronous round of replication. Four isogenic strains were made lysogenic for lambdaind(-) and for phage Mu-1, with Mu-1 integrated into a different chromosomal location in each strain. Cultures were starved for amino acids to allow completion of chromosome replication cycles and then starved for thymine in the presence of amino acids, and a synchronous cycle of replication was initiated by the addition of thymine. Samples were exposed to radioactive thymidine at intervals, deoxyribonucleic acid was extracted, and the rate of marker replication was determined by deoxyribonucleic acid-deoxyribonucleic acid hybridization to filters containing Mu-1, lambda, and E. coli deoxyribonucleic acid. The results confirm that the origin of replication is near ilv. The travel times of the replication forks, calculated from the data obtained for cultures with doubling times of approximately 40 and 61 min, are 40 and 52 min, respectively. |
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Authors:
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J Louarn; M Funderburgh; R E Bird |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of bacteriology Volume: 120 ISSN: 0021-9193 ISO Abbreviation: J. Bacteriol. Publication Date: 1974 Oct |
Date Detail:
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Created Date: 1975-01-13 Completed Date: 1975-01-13 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 2985120R Medline TA: J Bacteriol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1-5 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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metabolism Chromosome Mapping* Coliphages DNA Replication* DNA Viruses DNA, Bacterial / biosynthesis* Escherichia coli / metabolism* Glucose / metabolism Lysogeny Nucleic Acid Hybridization Spectrophotometry Thymidine / metabolism Thymine / metabolism Tritium |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/DNA, Bacterial; 10028-17-8/Tritium; 50-89-5/Thymidine; 50-99-7/Glucose; 65-71-4/Thymine |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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