Document Detail

Monovalent cations mediate formation of native tertiary structure of the Tetrahymena thermophila ribozyme.
MedLine Citation:
PMID:  12434149     Owner:  NLM     Status:  MEDLINE    
The formation of individual tertiary contacts of the Tetrahymena L-21 Sca I ribozyme has been monitored by hydroxyl radical footprinting and its global conformation by analytical ultracentrifugation as a function of monovalent ion concentration in the absence of divalent ions. Advanced methods of data analysis, which allow the hydroxyl radical reactivity of every nucleotide to be quantified, permit monitoring of each and every structural element of the RNA. Monovalent ion-mediated global compaction of the ribozyme is accompanied by the formation of native tertiary contacts; most native tertiary contacts are evident except several that are located near where divalent ions are observed in crystallographic structures. Non-native tertiary contacts are also observed at low but not high concentrations of monovalent ions. In light of recent studies that have shown that the presence of monovalent ions greatly accelerates the Mg2+-dependent folding of the Tetrahymena ribozyme, the present studies suggest that Na+ concentration changes not only the starting position of the RNA on its folding funnel but also pushes it deep into the well by forming native tertiary contacts and, thus, favoring fast and correct folding pathways.
Keiji Takamoto; Qin He; Stephanie Morris; Mark R Chance; Michael Brenowitz
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Nature structural biology     Volume:  9     ISSN:  1072-8368     ISO Abbreviation:  Nat. Struct. Biol.     Publication Date:  2002 Dec 
Date Detail:
Created Date:  2002-11-26     Completed Date:  2002-12-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9421566     Medline TA:  Nat Struct Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  928-33     Citation Subset:  IM    
Department of Physiology and Biophysics, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461, USA.
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MeSH Terms
Base Sequence
Cations, Monovalent
Hydroxyl Radical
Models, Molecular
Molecular Sequence Data
Nucleic Acid Conformation
RNA, Catalytic / chemistry*
Ribonucleotides / analysis
Sodium / pharmacology
Tetrahymena thermophila / enzymology*
Reg. No./Substance:
0/Cations, Monovalent; 0/RNA, Catalytic; 0/Ribonucleotides; 3352-57-6/Hydroxyl Radical; 7440-23-5/Sodium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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