Document Detail


Monovalent cation composition and ATP and lipid content of irreversibly sickled cells.
MedLine Citation:
PMID:  647122     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Discontinuous Stractan gradients were used to separate heterogeneous populations of sickle cells into discrete subpopulations containing varying proportions of reticulocytes, mature discoid cells, and irreversibly sickled cells (ISC). The improved homogeneity of these preparations, together with an enhanced yield of ISC, allowed us to distinguish effects of cell maturation from those of irreversible sickling. With these cell preparations we have begun to define physical properties of ISC. We confirmed the marked abnormalities in cation composition of native ISC. Measurements of ATP in ISC did not substantiate prior reports of ATP deficits. Finally, no evidence for substantial loss of membrane lipids during the process of ISC formation could be demonstrated.
Authors:
M R Clark; R C Unger; S B Shohet
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Blood     Volume:  51     ISSN:  0006-4971     ISO Abbreviation:  Blood     Publication Date:  1978 Jun 
Date Detail:
Created Date:  1978-07-24     Completed Date:  1978-07-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7603509     Medline TA:  Blood     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1169-78     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphate / blood*
Anemia, Sickle Cell / blood*
Cations, Monovalent
Centrifugation, Density Gradient
Cholesterol / blood
Erythrocytes, Abnormal / metabolism*
Humans
Lipids / blood*
Phosphorus / blood
Potassium / blood
Reticulocytes
Sodium / blood
Chemical
Reg. No./Substance:
0/Cations, Monovalent; 0/Lipids; 56-65-5/Adenosine Triphosphate; 57-88-5/Cholesterol; 7440-09-7/Potassium; 7440-23-5/Sodium; 7723-14-0/Phosphorus

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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