| Monounsaturated trans fatty acids, elaidic acid and trans-vaccenic acid, metabolism and incorporation in phospholipid molecular species in hepatocytes. | |
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MedLine Citation:
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PMID: 10088200 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The incorporation of [14C]elaidic acid (trans18:1(n-9)) in phosphatidylcholine and phosphatidylethanolamine molecular species in isolated rat liver cells has been studied, and the results compared with the incorporation, previously published (B. Woldseth et al. Biochim Biophys Acta 1993; 1167: 296-302), of [14C]palmitic acid (16:0) and [14C]stearic acid (18:0) and with that of [14C]oleic acid (cis18:1(n-9)). The pattern of incorporation in phospholipid molecular species is similar to that of [14C]stearic acid and different from that of [14C]palmitic acid. In phosphatidylcholine [14C]trans18:1-18:2 and [14C]trans18:1-20:4 were the most abundant species, and in phosphatidylethanolamine [14C]trans18:1-20:4 was the predominant species. With increasing concentration of [14C]elaidic acid increasing amounts of [14C]trans18:1-[14C]trans18:1 were found. The total incorporation in phospholipids was less than that of [14C]stearic acid, but more than that of [14C]palmitic acid. The distribution in percent of [14C]elaidic acid in phospholipid classes was 8.8% in phosphatidylinositol, 1.8% in phosphatidylserine, 59.1% in phosphatidylcholine and 30.3% in phosphatidylethanolamine with 0.1 mmol l-1 substrate concentration. More [14C]elaidic acid than [14C]palmitic acid or [14C]stearic acid was oxidized. The incorporation in phospholipids of [14C]elaidic acid was very different from that of [14C]oleic acid. The main species with [14C]oleic acid were 16:0-[14C]cis18:1 in phosphatidylcholine, and [14C]cis18:1-20:4 in phosphatidylethanolamine. In some experiments [14C]18:2(n-6) was incubated together with unlabelled elaidic or unlabelled trans-vaccenic acid (trans18:1(n-7)). In these experiments, more trans18:1-18:2 was formed from elaidic acid than from trans-vaccenic acid, especially in phosphatidylethanolamine. |
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Authors:
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B Woldseth; K Retterstøl; B O Christophersen |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Scandinavian journal of clinical and laboratory investigation Volume: 58 ISSN: 0036-5513 ISO Abbreviation: Scand. J. Clin. Lab. Invest. Publication Date: 1998 Dec |
Date Detail:
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Created Date: 1999-05-21 Completed Date: 1999-05-21 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0404375 Medline TA: Scand J Clin Lab Invest Country: NORWAY |
Other Details:
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Languages: eng Pagination: 635-45 Citation Subset: IM |
Affiliation:
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Institute of Clinical Biochemistry, University of Oslo, Norway. berit.woldseth@rh.uio.no |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Carbon Radioisotopes / diagnostic use Chromatography, Gas Esterification Fatty Acids, Monounsaturated / metabolism* Linoleic Acid / metabolism Liver / cytology, metabolism* Male Oleic Acid / metabolism* Oleic Acids / metabolism* Oxidation-Reduction Palmitic Acid / metabolism Phosphatidylcholines / metabolism Phosphatidylethanolamines / metabolism Rats Rats, Wistar Stearic Acids / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Carbon Radioisotopes; 0/Fatty Acids, Monounsaturated; 0/Oleic Acids; 0/Phosphatidylcholines; 0/Phosphatidylethanolamines; 0/Stearic Acids; 112-79-8/elaidic acid; 112-80-1/Oleic Acid; 143-25-9/11-octadecenoic acid; 2197-37-7/Linoleic Acid; 57-10-3/Palmitic Acid; 57-11-4/stearic acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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