| Monounsaturated fatty acyl-coenzyme A is predictive of atherosclerosis in human apoB-100 transgenic, LDLr-/- mice. | |
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MedLine Citation:
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PMID: 17277381 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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ACAT2, the enzyme responsible for the formation of cholesteryl esters incorporated into apolipoprotein B-containing lipoproteins by the small intestine and liver, forms predominantly cholesteryl oleate from acyl-CoA and free cholesterol. The accumulation of cholesteryl oleate in plasma lipoproteins has been found to be predictive of atherosclerosis. Accordingly, a method was developed in which fatty acyl-CoA subspecies could be extracted from mouse liver and quantified. Analyses were performed on liver tissue from mice fed one of four diets enriched with one particular type of dietary fatty acid: saturated, monounsaturated, n-3 polyunsaturated, or n-6 polyunsaturated. We found that the hepatic fatty acyl-CoA pools reflected the fatty acid composition of the diet fed. The highest percentage of fatty acyl-CoAs across all diet groups was in monoacyl-CoAs, and values were 36% and 46% for the n-3 and n-6 polyunsaturated diet groups and 55% and 62% in the saturated and monounsaturated diet groups, respectively. The percentage of hepatic acyl-CoA as oleoyl-CoA was also highly correlated to liver cholesteryl ester, plasma cholesterol, LDL molecular weight, and atherosclerosis extent. These data suggest that replacing monounsaturated with polyunsaturated fat can benefit coronary heart disease by reducing the availability of oleoyl-CoA in the substrate pool of hepatic ACAT2, thereby reducing cholesteryl oleate secretion and accumulation in plasma lipoproteins. |
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Authors:
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Thomas A Bell; Martha D Wilson; Kathryn Kelley; Janet K Sawyer; Lawrence L Rudel |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2007-02-04 |
Journal Detail:
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Title: Journal of lipid research Volume: 48 ISSN: 0022-2275 ISO Abbreviation: J. Lipid Res. Publication Date: 2007 May |
Date Detail:
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Created Date: 2007-05-02 Completed Date: 2007-06-22 Revised Date: 2007-12-03 |
Medline Journal Info:
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Nlm Unique ID: 0376606 Medline TA: J Lipid Res Country: United States |
Other Details:
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Languages: eng Pagination: 1122-31 Citation Subset: IM |
Affiliation:
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Department of Pathology, Section on Lipid Sciences, Wake Forest University School of Medicine, Winston-Salem, NC 27157, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acyl Coenzyme A
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metabolism* Animal Feed Animals Apolipoprotein B-100 / genetics, metabolism* Atherosclerosis / genetics, metabolism*, pathology* Biological Markers Cholesterol / blood Cholesterol Esters / metabolism Coenzyme A Ligases / metabolism Fatty Acids / metabolism Humans Lipid Metabolism Liver / drug effects, metabolism Mice Mice, Transgenic RNA, Messenger / genetics Receptors, LDL / deficiency*, genetics, metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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AT-0027820/AT/NCCAM NIH HHS; HL-24736/HL/NHLBI NIH HHS; HL-49373/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Acyl Coenzyme A; 0/Apolipoprotein B-100; 0/Biological Markers; 0/Cholesterol Esters; 0/Fatty Acids; 0/RNA, Messenger; 0/Receptors, LDL; 57-88-5/Cholesterol; EC 6.2.1.-/Coenzyme A Ligases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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