Document Detail


Monoterpenes in the glandular trichomes of tomato are synthesized from a neryl diphosphate precursor rather than geranyl diphosphate.
MedLine Citation:
PMID:  19487664     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We identified a cis-prenyltransferase gene, neryl diphosphate synthase 1 (NDPS1), that is expressed in cultivated tomato (Solanum lycopersicum) cultivar M82 type VI glandular trichomes and encodes an enzyme that catalyzes the formation of neryl diphosphate from isopentenyl diphosphate and dimethylallyl diphosphate. mRNA for a terpene synthase gene, phellandrene synthase 1 (PHS1), was also identified in these glands. It encodes an enzyme that uses neryl diphosphate to produce beta-phellandrene as the major product as well as a variety of other monoterpenes. The profile of monoterpenes produced by PHS1 is identical with the monoterpenes found in type VI glands. PHS1 and NDPS1 map to chromosome 8, and the presence of a segment of chromosome 8 derived from Solanum pennellii LA0716 causes conversion from the M82 gland monoterpene pattern to that characteristic of LA0716 plants. The data indicate that, contrary to the textbook view of geranyl diphosphate as the "universal" substrate of monoterpene synthases, in tomato glands neryl diphosphate serves as a precursor for the synthesis of monoterpenes.
Authors:
Anthony L Schilmiller; Ines Schauvinhold; Matthew Larson; Richard Xu; Amanda L Charbonneau; Adam Schmidt; Curtis Wilkerson; Robert L Last; Eran Pichersky
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2009-06-01
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  106     ISSN:  1091-6490     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  2009 Jun 
Date Detail:
Created Date:  2009-07-01     Completed Date:  2009-09-28     Revised Date:  2010-09-27    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  10865-70     Citation Subset:  IM    
Affiliation:
Department of Biochemistry and Molecular Biology, Research Technology Support Facility, Michigan State University, East Lansing, MI 48824-1319, USA.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/FJ797956;  FJ797957
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MeSH Terms
Descriptor/Qualifier:
Alkyl and Aryl Transferases / genetics,  metabolism
Biosynthetic Pathways
Cloning, Molecular
DNA, Complementary / chemistry,  genetics
Dimethylallyltranstransferase / genetics,  metabolism
Diphosphates / chemistry,  metabolism
Diterpenes / chemistry,  metabolism
Gas Chromatography-Mass Spectrometry
Gene Expression Regulation, Plant
Gene Library
Hemiterpenes / chemistry,  metabolism
Kinetics
Lycopersicon esculentum / enzymology*,  genetics,  metabolism*
Molecular Sequence Data
Molecular Structure
Monoterpenes / analysis,  chemistry,  metabolism*
Organophosphorus Compounds / chemistry,  metabolism
Plant Epidermis / enzymology,  metabolism
Plant Proteins / genetics,  metabolism
Polyisoprenyl Phosphates / chemistry,  metabolism*
Reverse Transcriptase Polymerase Chain Reaction
Sequence Analysis, DNA
Substrate Specificity
Chemical
Reg. No./Substance:
0/DNA, Complementary; 0/Diphosphates; 0/Diterpenes; 0/Hemiterpenes; 0/Monoterpenes; 0/Organophosphorus Compounds; 0/Plant Proteins; 0/Polyisoprenyl Phosphates; 0/geranyl diphosphate; 358-71-4/isopentenyl pyrophosphate; 358-72-5/3,3-dimethylallyl pyrophosphate; 763-10-0/geranyl pyrophosphate; 99-83-2/alpha phellandrene; EC 2.5.-/Alkyl and Aryl Transferases; EC 2.5.1.-/terpene synthase; EC 2.5.1.1/Dimethylallyltranstransferase
Comments/Corrections
Comment In:
Proc Natl Acad Sci U S A. 2009 Jun 30;106(26):10402-3   [PMID:  19553206 ]

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