| Monosialogangliosides of rabbit skeletal muscle. Characterization of N-acetylneuraminosyl lacto-N-noroctaosyl ceramide. | |
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MedLine Citation:
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PMID: 7251579 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Rabbit skeletal muscle contained 28.4 nanomol/g wet weight of lipid-bound sialic acid, and 73.4% of the total lipid-bound sialic acid was recovered in a monosialoganglioside fraction. Monosialoganglioside components were isolated and the structures were determined by exoglycosidase treatment and permethylation analysis. The major monosialoganglioside was GM3 (73.2% of monosialogangliosides), and gangliosides of the lacto-series with at least three repeating units of lactosamine, Gal(beta, 1-4)GlcNAc(beta, 1-3), were identified. A newly found ganglioside was N-acetylneuraminosyl lacto-N-noroctaosyl ceramide, NeuAc(alpha, 2-3)Gal(beta, 1-4)GlcNAc (beta, 1-3)Gal(beta, 1-4)GlcNAc(beta, 1-3)Gal(beta, 1-4)GlcNAc(beta, 1-3)Gal(beta, 1-4)Glc(beta, 1-1)ceramide. The amounts of lacto-series gangliosides in the monosialoganglioside fraction were as follows: sialosyl lacto-N-neotetraosyl ceramide (9.8%), sialosyl lacto-N-norhexaosyl ceramide (11.9%) and sialosyl lacto-N-noroctaosyl ceramide (5.1%). N-Glycolylneuraminic acid was found in sialosyl lacto-N-neotetraosyl ceramide and sialosyl lacto-N-norhexaosyl ceramide but the amount was less than 0.2%. The mobilities of lacto-series gangliosides were compared with those of ganglio-series gangliosides. Fatty acid and long chain base compositions were also determined. |
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Authors:
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M Iwamori; Y Nagai |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of biochemistry Volume: 89 ISSN: 0021-924X ISO Abbreviation: J. Biochem. Publication Date: 1981 Apr |
Date Detail:
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Created Date: 1981-09-15 Completed Date: 1981-09-15 Revised Date: 2007-12-19 |
Medline Journal Info:
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Nlm Unique ID: 0376600 Medline TA: J Biochem Country: JAPAN |
Other Details:
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Languages: eng Pagination: 1253-64 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Carbohydrate Sequence Carbohydrates / analysis Fatty Acids / analysis Gangliosides / isolation & purification* Glycosphingolipids / isolation & purification* Lactosylceramides / isolation & purification* Methylation Muscles / analysis* Neuraminidase / metabolism Rabbits |
| Chemical | |
Reg. No./Substance:
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0/Carbohydrates; 0/Fatty Acids; 0/Gangliosides; 0/Glycosphingolipids; 0/Lactosylceramides; 77700-05-1/N-acetylneuraminosyl lacto-N-noroctaosyl ceramide; EC 3.2.1.18/Neuraminidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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