Document Detail


Monosaccharides versus PEG-functionalized NPs: influence in the cellular uptake.
MedLine Citation:
PMID:  22214244     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Magnetic nanoparticles (NPs) hold great promise for biomedical applications. The core composition and small size of these particles produce superparamagnetic behavior, thus facilitating their use in magnetic resonance imaging and magnetically induced therapeutic hyperthermia. However, the development and control of safe in vivo applications for NPs call for the study of cell-NP interactions and cell viability. Furthermore, as for most biotechnological applications, it is desirable to prevent unspecific cell internalization of these particles. It is also crucial to understand how the surface composition of the NPs affects their internalization capacity. Here, through accurate control over unspecific protein adsorption, size distribution, grafting density, and an extensive physicochemical characterization, we correlated the cytotoxicity and cellular uptake mechanism of 6 nm magnetic NPs coated with several types and various densities of biomolecules, such as glucose, galactose, and poly(ethylene glycol). We found that the density of the grafted molecule was crucial to prevent unspecific uptake of NPs by Vero cells. Surprisingly, the glucose-coated NPs described here showed cellular uptake as a result of lipid raft instead of clathrin-mediated cellular internalization. Moreover, these glucose-functionalized NPs could be one of the first examples of NPs being endocytosed by caveolae that finally end up in the lysosomes. These results reinforce the use of simple carbohydrates as an alternative to PEG molecules for NPs functionalization when cellular uptake is required.
Authors:
María Moros; Bruno Hernáez; Elina Garet; Jorge T Dias; Berta Sáez; Valeria Grazú; Africa González-Fernández; Covadonga Alonso; Jesús M de la Fuente
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-01-09
Journal Detail:
Title:  ACS nano     Volume:  6     ISSN:  1936-086X     ISO Abbreviation:  ACS Nano     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-02-28     Completed Date:  2012-06-19     Revised Date:  2012-08-24    
Medline Journal Info:
Nlm Unique ID:  101313589     Medline TA:  ACS Nano     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1565-77     Citation Subset:  IM    
Affiliation:
Biofunctionalization of Nanoparticles and Surfaces (BioNanoSurf), Instituto de Nanociencia de Aragón, Universidad de Zaragoza, Mariano Esquillor, s/n, 50018 Zaragoza, Spain.
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MeSH Terms
Descriptor/Qualifier:
Adsorption
Animals
Biological Transport
HeLa Cells
Humans
Mice
Monosaccharides / chemistry*
Nanoparticles / chemistry*
Particle Size
Polyethylene Glycols / chemistry*
Surface Properties
Chemical
Reg. No./Substance:
0/Monosaccharides; 0/Polyethylene Glycols
Comments/Corrections
Comment In:
Nanomedicine (Lond). 2012 Jun;7(6):803   [PMID:  22734638 ]

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