| Monomer plasmid DNA transforms Streptococcus pneumoniae. | |
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MedLine Citation:
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PMID: 6938758 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The covalently closed (CC) monomer form of plasmid pMV158 was found to transform pneumococcus (Streptococcus pneumoniae) and to do so with two-hit kinetics. The evidence came from analysis of the behavior of the transforming activity in fractions from preparative gel electrophoresis. Activity in the first major peak to elute (i) co-eluted with monomer CC as detected on analytical gels, (ii) banded as CC in dye-buoyancy gradients, (iii) sedimented with the velocity expected for monomer CC, and (iv) gave two-hit kinetics as functions of both concentration and time of exposure of the cells to DNA. A second major peak of activity behaved physically as though mostly due to dimer CC forms and gave single-hit response curves. Because almost no dimer was detectable optically on analytical gels of starting preparations, its specific activity was high relative to that of the monomers. |
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Authors:
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C W Saunders; W R Guild |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Molecular & general genetics : MGG Volume: 181 ISSN: 0026-8925 ISO Abbreviation: Mol. Gen. Genet. Publication Date: 1981 |
Date Detail:
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Created Date: 1981-06-23 Completed Date: 1981-06-23 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0125036 Medline TA: Mol Gen Genet Country: GERMANY, WEST |
Other Details:
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Languages: eng Pagination: 57-62 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Centrifugation, Density Gradient DNA, Bacterial / genetics, isolation & purification DNA, Circular / genetics, isolation & purification Electrophoresis, Agar Gel Kinetics Plasmids* Streptococcus pneumoniae / genetics* Transformation, Genetic* |
| Grant Support | |
ID/Acronym/Agency:
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1 T32 GM07754/GM/NIGMS NIH HHS; GM21887/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/DNA, Bacterial; 0/DNA, Circular |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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