Document Detail

Monocytic differentiation and synthesis of proteins associated with apoptosis in human leukemia U-937 cells acquiring resistance to vincristine.
MedLine Citation:
PMID:  8698136     Owner:  NLM     Status:  MEDLINE    
Human leukemia U-937/WT cells were exposed to stepwise increased concentrations of Vincristine so that Vincristine-resistant cell sublines (termed U-937/RV) were developed. Established U-937/RV cell sublines have continuously propagated over a year, both in absence and presence of VCR, and have demonstrated similar features. In contrast to U-937/WT cells, U-937/RV cells have longer doubling time, and are more differentiated as determined by appearance of distinct morphological features and synthesis of mRNA that codes for the monocyte colony-stimulating factor-1 receptor (c-fms). Both apoptosis-suppressing Bcl-2 and Bcl-XL proteins were undectable in U-937/WT cells, whereas Bcl-2 was nearly detectable and Bcl-XL readily detectable in U-937/RV cells. The apoptosis-promoting Bax protein was also absent in U-937/WT cells and readily detected in U-937/RV cells. Vincristine-resistant cells with different levels of resistance synthesize similar levels of c-fms mRNA and Bax protein. Finally, unlike U-937/WT cells, U-937/RV cells have no ability to induce tumors when xenografted in immunodeficient mice. The findings collectively suggest that development of resistance to Vincristine in U-937/WT cells may correlate with cell differentiation and synthesis of proteins that regulate apoptosis.
P Pantazis; D Chatterjee; Z Han; J Wyche; A DeJesus; B Giovanella
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  European journal of haematology     Volume:  57     ISSN:  0902-4441     ISO Abbreviation:  Eur. J. Haematol.     Publication Date:  1996 Jul 
Date Detail:
Created Date:  1996-08-30     Completed Date:  1996-08-30     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8703985     Medline TA:  Eur J Haematol     Country:  DENMARK    
Other Details:
Languages:  eng     Pagination:  79-86     Citation Subset:  IM    
Stehlin Foundation for Cancer Research, St Joseph Hospital, Houston, TX 77003, USA.
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MeSH Terms
Cell Differentiation
Cell Division
Drug Resistance, Neoplasm*
Gene Expression
Leukemia / metabolism,  pathology*
Mice, Nude
Monocytes / metabolism,  pathology*
Neoplasm Transplantation
Protein Biosynthesis*
Proto-Oncogene Proteins / biosynthesis
Proto-Oncogene Proteins c-bcl-2
RNA, Messenger / biosynthesis
Receptor, Macrophage Colony-Stimulating Factor / genetics
Tumor Cells, Cultured
Vincristine / pharmacology*
bcl-2-Associated X Protein
bcl-X Protein
Reg. No./Substance:
0/BAX protein, human; 0/BCL2L1 protein, human; 0/Bax protein, mouse; 0/Bcl2l1 protein, mouse; 0/Proto-Oncogene Proteins; 0/Proto-Oncogene Proteins c-bcl-2; 0/RNA, Messenger; 0/bcl-2-Associated X Protein; 0/bcl-X Protein; 57-22-7/Vincristine; EC, Macrophage Colony-Stimulating Factor

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