| Monocytic differentiation and synthesis of proteins associated with apoptosis in human leukemia U-937 cells acquiring resistance to vincristine. | |
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MedLine Citation:
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PMID: 8698136 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Human leukemia U-937/WT cells were exposed to stepwise increased concentrations of Vincristine so that Vincristine-resistant cell sublines (termed U-937/RV) were developed. Established U-937/RV cell sublines have continuously propagated over a year, both in absence and presence of VCR, and have demonstrated similar features. In contrast to U-937/WT cells, U-937/RV cells have longer doubling time, and are more differentiated as determined by appearance of distinct morphological features and synthesis of mRNA that codes for the monocyte colony-stimulating factor-1 receptor (c-fms). Both apoptosis-suppressing Bcl-2 and Bcl-XL proteins were undectable in U-937/WT cells, whereas Bcl-2 was nearly detectable and Bcl-XL readily detectable in U-937/RV cells. The apoptosis-promoting Bax protein was also absent in U-937/WT cells and readily detected in U-937/RV cells. Vincristine-resistant cells with different levels of resistance synthesize similar levels of c-fms mRNA and Bax protein. Finally, unlike U-937/WT cells, U-937/RV cells have no ability to induce tumors when xenografted in immunodeficient mice. The findings collectively suggest that development of resistance to Vincristine in U-937/WT cells may correlate with cell differentiation and synthesis of proteins that regulate apoptosis. |
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Authors:
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P Pantazis; D Chatterjee; Z Han; J Wyche; A DeJesus; B Giovanella |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: European journal of haematology Volume: 57 ISSN: 0902-4441 ISO Abbreviation: Eur. J. Haematol. Publication Date: 1996 Jul |
Date Detail:
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Created Date: 1996-08-30 Completed Date: 1996-08-30 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 8703985 Medline TA: Eur J Haematol Country: DENMARK |
Other Details:
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Languages: eng Pagination: 79-86 Citation Subset: IM |
Affiliation:
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Stehlin Foundation for Cancer Research, St Joseph Hospital, Houston, TX 77003, USA. ppant@pipeline.com |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis* Cell Differentiation Cell Division Drug Resistance, Neoplasm* Gene Expression Humans Leukemia / metabolism, pathology* Mice Mice, Nude Monocytes / metabolism, pathology* Neoplasm Transplantation Protein Biosynthesis* Proto-Oncogene Proteins / biosynthesis Proto-Oncogene Proteins c-bcl-2 RNA, Messenger / biosynthesis Receptor, Macrophage Colony-Stimulating Factor / genetics Tumor Cells, Cultured Vincristine / pharmacology* bcl-2-Associated X Protein bcl-X Protein |
| Chemical | |
Reg. No./Substance:
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0/BAX protein, human; 0/BCL2L1 protein, human; 0/Bax protein, mouse; 0/Bcl2l1 protein, mouse; 0/Proto-Oncogene Proteins; 0/Proto-Oncogene Proteins c-bcl-2; 0/RNA, Messenger; 0/bcl-2-Associated X Protein; 0/bcl-X Protein; 57-22-7/Vincristine; EC 2.7.10.1/Receptor, Macrophage Colony-Stimulating Factor |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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