| Monocytes P2X7 purinergic receptor is modulated by glatiramer acetate in multiple sclerosis. | |
| | |
MedLine Citation:
|
PMID: 22370183 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
The aim of this study is to investigate the expression of P2X7R, IL-1beta and the ATP activity modulating ecto-apyrase CD39 on peripheral blood monocytes of MS patients and to observe the possible effects of Glatiramer Acetate (GA) on such expression. Twelve RR treatment-free MS patients were selected and peripheral blood monocytes were obtained. The expression of P2X7R, IL-1beta and CD39 on monocytes was investigated by qrt-PCR. The in vitro effects of GA on the expression of monocytes stimulated with BzATP (a potent P2X7R agonist)-were evaluated. Ten healthy donors (HDs) were similarly studied. Finally, 5 MS patients were given GA therapy and the monocytes obtained before treatment, after 3 and 12months of GA treatment were similarly investigated. No differences were found in P2X7R, IL-1beta and CD39 expression between patients and controls. In MS Bz-ATP stimulated monocytes, GA pre-conditioning clearly downregulated P2X7R (p=0.003) but IL-1beta expression also showed a decreasing trend (p=0.07). Conversely, CD39 showed an increasing trend (p=0.07). Similar evidence was found in HDs. GA in vivo treatment induced a reduction in the expression that was clear for P2X7R and CD39 (p<0.05) but only not significant for IL-1beta after 12months of treatment. Monocytes from both MS and control subjects express P2X7R, IL-1beta and CD39, and GA seems to interfere with such expression. |
| | |
Authors:
|
Mariantonietta Caragnano; Paola Tortorella; Alessandra Bergami; Maddalena Ruggieri; Paolo Livrea; Luigi Maria Specchio; Gianvito Martino; Maria Trojano; Roberto Furlan; Carlo Avolio |
Related Documents
:
|
9446603 - A novel nk-2-related transcription factor associated with human fetal liver and hepatoc... 8631153 - Expression of hepatocyte-enriched nuclear transcription factors in mouse liver tumours. 18430783 - Zfp64 participates in notch signaling and regulates differentiation in mesenchymal cells. |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-2-25 |
Journal Detail:
|
Title: Journal of neuroimmunology Volume: - ISSN: 1872-8421 ISO Abbreviation: - Publication Date: 2012 Feb |
Date Detail:
|
Created Date: 2012-2-28 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8109498 Medline TA: J Neuroimmunol Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
Copyright © 2012 Elsevier B.V. All rights reserved. |
Affiliation:
|
Department of Medical and Occupational Sci., University of Foggia, Italy. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Interrelated effects of dihomo-?-linolenic and arachidonic acids, and sesamin on hepatic fatty acid ...
Next Document: On display with transparent conducting films.