| Monocyte phenotype and cytokine production profiles are dysregulated by short-duration spaceflight. | |
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MedLine Citation:
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PMID: 21888268 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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INTRODUCTION: Immune system dysregulation has been demonstrated to occur during and immediately following spaceflight. As the initial bias and magnitude for an immune response is heavily influenced by monocyte/macrophage secreted cytokines, this study investigated monocyte phenotype and cytokine production patterns following short-duration spaceflight. METHODS: Secreted cytokine profiles were examined by cytometric bead array analysis of culture supernatants following whole blood culture activation with LPS or PMA+ionomycin. Nine short-duration Space Shuttle crewmembers participated in this study. RESULTS: Peripheral monocyte percentages were unaltered postflight. Constitutive monocyte expression of both CD62L and HLA-DR was reduced following spaceflight in a mission-specific fashion. Loss of either molecule indicates a functional disability of monocytes, either by inhibition of adhesion and tissue migration (CD62L) or by impaired antigen presentation (HLA-DR). Following LPS stimulation of monocytes, postflight expression of IL-6, TNFalpha, and IL-10 were significantly reduced (by 43%, 44%, and 41%, respectively) and expression of IL-1b was elevated (65%). IL-8 production was either elevated or reduced in a mission-specific fashion. Following PMA+ionomycin stimulation of all leukocyte populations, only expression of IL-6 was significantly reduced postflight. DISCUSSION: These data indicate that changes in monocyte constitutive phenotype and inflammatory cytokine production occur following short-duration spaceflight, which may impact overall crewmember immunocompetence. Also, monocyte/macrophage function may be highly sensitive to mission specific parameters. |
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Authors:
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Brian Crucian; Raymond Stowe; Heather Quiriarte; Duane Pierson; Clarence Sams |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Aviation, space, and environmental medicine Volume: 82 ISSN: 0095-6562 ISO Abbreviation: Aviat Space Environ Med Publication Date: 2011 Sep |
Date Detail:
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Created Date: 2011-09-05 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7501714 Medline TA: Aviat Space Environ Med Country: United States |
Other Details:
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Languages: eng Pagination: 857-62 Citation Subset: IM; S |
Affiliation:
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NASA Johnson Space Center, 2101 NASA Parkway, Mail Code SK, Houston, TX 77058, USA. brian.crucian-1@nasa.gov |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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