Document Detail

Monocyte-derived growth factors in asbestos-induced interstitial fibrosis.
MedLine Citation:
PMID:  2473896     Owner:  NLM     Status:  MEDLINE    
Asbestos-induced pulmonary fibrosis is thought to result from a series of cellular interactions involving the alveolar macrophage and the lung fibroblast. Although a dose-response relation has been established between asbestos exposure and the development of interstitial fibrosis, the majority of workers exposed even to high concentrations of asbestos do not develop radiographically evident interstitial fibrosis. As a means of understanding the pathogenesis of asbestos-induced fibrosis and the differential host response to the asbestos fiber, we studied the production of monocyte-derived growth factors in subjects with asbestosis (N = 5) and normal exposure-matched controls (N = 5). Under unstimulated culture conditions, the conditioned medium (CM) from monocytes from control subjects had a greater proliferative effect on fibroblasts than monocyte CM from patients with asbestosis. With CM from concanavalin A-stimulated monocytes, the proliferative response of the fibroblast was similar in those with asbestosis and control subjects. Freshly isolated monocytes from both normal subjects and patients with asbestosis did not express the gene for the B chain of platelet-derived growth factor (PDGF) as evaluated by hybridization with a PDGF-specific human cDNA probe. After 20 hr of culture in concanavalin A, monocytes from normal subjects expressed the gene for the B chain of PDGF while, under the same culture conditions, none of the monocyte preparations from patients with asbestosis produced mRNA for this growth factor. These data indicate that peripheral blood monocytes from patients with asbestosis compared to exposure-matched controls have a reduced capacity to produce growth factor activity as measured by mitogenic activity and mRNA levels for the B chain of PDGF. These results suggest that peripheral blood monocytes from patients with asbestosis may comprise a less mature population of cells compared to exposure-matched controls.
D A Schwartz; L Rosenstock; J G Clark
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Environmental research     Volume:  49     ISSN:  0013-9351     ISO Abbreviation:  Environ. Res.     Publication Date:  1989 Aug 
Date Detail:
Created Date:  1989-08-29     Completed Date:  1989-08-29     Revised Date:  2006-10-30    
Medline Journal Info:
Nlm Unique ID:  0147621     Medline TA:  Environ Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  283-94     Citation Subset:  IM    
Pulmonary and Critical Care Medicine Division, Fred Hutchinson Cancer Research Center, Seattle, Washington.
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MeSH Terms
Asbestos / adverse effects*
Asbestosis / blood
Cell Division / drug effects
Cells, Cultured
Growth Substances / blood*,  isolation & purification,  pharmacology
Monocytes / drug effects,  metabolism*
Nucleic Acid Hybridization
Occupational Diseases / blood,  etiology*
Pulmonary Fibrosis / blood,  etiology*
RNA / genetics,  isolation & purification
Reference Values
Reg. No./Substance:
0/Growth Substances; 1332-21-4/Asbestos; 63231-63-0/RNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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