Document Detail


Monoclonality and surface lesion-specific microsatellite alterations in premalignant and malignant neoplasia of uterine cervix: a local field effect of genomic instability and clonal evolution.
MedLine Citation:
PMID:  9885979     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Invasive squamous carcinoma of the uterine cervix (CC) arises from sequential progression of low-grade (L) and high-grade (H) squamous intraepithelial lesions (SILs). In clinical observations, these lesions are frequently found as synchronous multiple foci. The nature and evolutionary mechanism of these lesions are largely unknown. We have performed allelotyping of three 3p markers (at 3p14, 3p22-24, and 3p25) on 22 LSILs and 15 HSILs microdissected from patients with multiple (n = 21) or uniform (n = 6) cervical lesions. The results were analyzed together with our previous allelotyping of 57 deeply invasive CCs. Loss of heterozygosity at one of the three markers was observed in 23%, 27%, and 31 % of LSILs, HSILs, and CCs, respectively. Frequent and early allelic loss was noted (in 30% of LSILs and 50% of HSILs) at 3p14, which may harbor tumor suppressor genes involved in early stages of cervical carcinogenesis. A high frequency of microsatellite alteration (MA) was found in LSIL (41%) and HSIL (67%) but not in CC (5.3%). In particular, MA was more frequently found in low-grade lesions in association with invasive cancers (75%, 6/8) than in those associated with SILs (29%, 4/14) (P < 0.05). Together with the finding of a monoclonal origin of premalignant and malignant cervical lesions, the present results allow us to propose a model of local field effect of genomic instability that progressively affects the clonal evolution of SIL of uterine cervix.
Authors:
T Y Chu; C Y Shen; H S Lee; H S Liu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Genes, chromosomes & cancer     Volume:  24     ISSN:  1045-2257     ISO Abbreviation:  Genes Chromosomes Cancer     Publication Date:  1999 Feb 
Date Detail:
Created Date:  1999-06-04     Completed Date:  1999-06-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9007329     Medline TA:  Genes Chromosomes Cancer     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  127-34     Citation Subset:  IM    
Affiliation:
Department of Obstetrics and Gynecology, Tri-Service General Hospital, Taiwan, Republic of China. tychu@email.gen.net.tw
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MeSH Terms
Descriptor/Qualifier:
Carcinoma in Situ / etiology,  genetics,  pathology
Carcinoma, Squamous Cell / etiology,  genetics,  pathology
Cervical Intraepithelial Neoplasia / etiology,  genetics,  pathology
Chromosome Mapping
Chromosomes, Human, Pair 3 / genetics
Clone Cells
DNA, Neoplasm / isolation & purification
DNA, Viral / isolation & purification
Female
Genetic Markers
Genotype
Humans
Loss of Heterozygosity
Microsatellite Repeats / genetics
Neoplasm Invasiveness / genetics,  pathology
Papillomaviridae / isolation & purification
Papillomavirus Infections / complications,  genetics,  pathology
Precancerous Conditions / etiology,  genetics*,  pathology
Trinucleotide Repeat Expansion / genetics*
Tumor Virus Infections / complications,  genetics,  pathology
Uterine Cervical Neoplasms / etiology,  genetics*,  pathology
Uterine Neoplasms / etiology,  genetics*,  pathology
Chemical
Reg. No./Substance:
0/DNA, Neoplasm; 0/DNA, Viral; 0/Genetic Markers

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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