| Monoclonal and polyclonal antibodies against porcine mitochondrial aspartate aminotransferase: their inhibition modes and application to enzyme immunoassay. | |
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MedLine Citation:
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PMID: 3297098 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Monoclonal and polyclonal antibodies against porcine mitochondrial aspartate aminotransferase (m-AST) were prepared in order to study their effect on the kinetics of the enzyme and their possible use as diagnostic reagents. The most stable hybridoma clone, designated MH-1, was selected and cultured for mass production of the monoclonal antibody MA-1. MA-1 was purified by affinity chromatography with m-AST as a ligand. The m-AST activity was inhibited uncompetitively by preincubation with MA-1, but preincubation with the polyclonal antibody uncompetitively by preincubation with MA-1, but preincubation with the polyclonal antibody raised in a rabbit resulted in noncompetitive inhibition of the enzyme. These results suggest the usefulness of a monoclonal antibody for studying the mechanism of catalysis. Sandwich enzyme immunoassay methods for m-AST using both polyclonal and monoclonal antibody-coated polystyrene balls were established and permitted the determination of porcine m-AST on the order of 10(-11) and 10(-10)M, respectively. |
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Authors:
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T Suzuki; Y Kishi; M Totani; H Kagamiyama; T Murachi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biotechnology and applied biochemistry Volume: 9 ISSN: 0885-4513 ISO Abbreviation: Biotechnol. Appl. Biochem. Publication Date: 1987 Apr |
Date Detail:
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Created Date: 1987-07-24 Completed Date: 1987-07-24 Revised Date: 2007-03-21 |
Medline Journal Info:
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Nlm Unique ID: 8609465 Medline TA: Biotechnol Appl Biochem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 170-80 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies* / isolation & purification Antibodies, Monoclonal* / isolation & purification Aspartate Aminotransferases / analysis*, immunology, metabolism Immunoenzyme Techniques Kinetics Mice Mice, Inbred Strains Mitochondria, Muscle / enzymology* Swine |
| Chemical | |
Reg. No./Substance:
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0/Antibodies; 0/Antibodies, Monoclonal; EC 2.6.1.1/Aspartate Aminotransferases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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