Document Detail

Monoclonal immunoglobulin of CLL lymphocytes detected by radioimmunofixation with 125I-protein A of Triton X-100 solubilized lymphocyte material.
MedLine Citation:
PMID:  7050389     Owner:  NLM     Status:  MEDLINE    
A sensitive radioimmunofixation method (detection limit 1 mg/1 for monoclonal IgM) utilizing 125I Protein A and immunofixation for demonstration of monoclonal immunoglobulin in Triton X-100 extracts of isolated chronic lymphocytic leukemia (CLL) lymphocytes is presented. Thirty-two untreated patients with clinically typical CLL were studied and the results compared to those obtained by immunofluorescence. Monoclonal IgM was detected with similar frequency by the two methods (69% and 75%), but IgD was detected less frequently by radioimmunofixation (25% and 40%). When the results obtained with the two methods were combined, all of the cases were immunoglobulin positive. Two complete IgM type M-components were demonstrated in two cases, an excess of seemingly free heavy chains was found in four cases, and an excess of seemingly free light chains in six cases.
T Plesner; M Wilken; O J Bjerrum; M Mørk Hansen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of clinical & laboratory immunology     Volume:  8     ISSN:  0141-2760     ISO Abbreviation:  J Clin Lab Immunol     Publication Date:  1982 Jun 
Date Detail:
Created Date:  1982-10-29     Completed Date:  1982-10-29     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  7808987     Medline TA:  J Clin Lab Immunol     Country:  ITALY    
Other Details:
Languages:  eng     Pagination:  137-41     Citation Subset:  IM    
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MeSH Terms
Antibodies, Monoclonal / analysis*
Blood Proteins / analysis
Electrophoresis, Agar Gel
Immunoglobulin M / analysis
Immunologic Techniques*
Iodine Radioisotopes
Leukemia, Lymphoid / analysis*
Lymphocytes / analysis*
Polyethylene Glycols / pharmacology
Staphylococcal Protein A / metabolism
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Blood Proteins; 0/Immunoglobulin M; 0/Immunoglobulins; 0/Iodine Radioisotopes; 0/M-proteins (Myeloma); 0/Polyethylene Glycols; 0/Staphylococcal Protein A

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