Document Detail


Monoclonal enzyme immunoassay measurement of estradiol and progesterone receptors in in vitro fertilization and spontaneous cycles.
MedLine Citation:
PMID:  1499845     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
So far, the few studies performed on endometrial steroid receptors in in vitro fertilization (IVF) cycles have been carried out by the dextran-coated charcoal (DCC) method. Despite widespread efforts to standardize the DCC assay method, assay results still vary and thus, data obtained in IVF cycles are controversial. In the present article, late luteal estradiol (E2) and progesterone (P) receptor (R) levels were measured by a new monoclonal enzyme immunoassay (EIA) method in endometrial samples obtained from 21 patients in an IVF program who received no embryo transfer (ET) after ovarian stimulation with follicle-stimulating hormone/human menopausal gonadotrophin/human chorionic gonadotrophin under pituitary suppression with buserelin. Plasma levels of E2, P and prolactin (PRL) were measured in three blood samples collected in the midluteal phase. Results were compared with those obtained in a control group of 21 spontaneous cycles. E2, P and PRL were significantly higher in stimulated than in spontaneous cycles. The level of E2R was decreased in endometrium in IVF stimulated cycles, but PR remained unchanged.
Authors:
J Balasch; F Rivera; I C Jové; J A Vanrell
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  European journal of obstetrics, gynecology, and reproductive biology     Volume:  45     ISSN:  0301-2115     ISO Abbreviation:  Eur. J. Obstet. Gynecol. Reprod. Biol.     Publication Date:  1992 Jul 
Date Detail:
Created Date:  1992-09-15     Completed Date:  1992-09-15     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0375672     Medline TA:  Eur J Obstet Gynecol Reprod Biol     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  113-7     Citation Subset:  IM    
Affiliation:
Department of Obstetrics and Gynecology, Faculty of Medicine, Hospital Clinic i Provincial, Barcelona, Spain.
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MeSH Terms
Descriptor/Qualifier:
Adult
Antibodies, Monoclonal*
Endometrium / chemistry*
Estradiol / blood
Female
Fertilization in Vitro*
Humans
Immunoenzyme Techniques*
Luteal Phase
Progesterone / blood
Prolactin / blood
Receptors, Estradiol / analysis*
Receptors, Progesterone / analysis*
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Receptors, Estradiol; 0/Receptors, Progesterone; 50-28-2/Estradiol; 57-83-0/Progesterone; 9002-62-4/Prolactin

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