Document Detail

Monoclonal antibody to galactosylceramide: discrimination of structural difference in the ceramide moiety.
MedLine Citation:
PMID:  2480915     Owner:  NLM     Status:  MEDLINE    
A mouse monoclonal antibody (mAb) was developed against monohexaosylceramide. This mAb differentially reacted on thin-layer chromatograms with 3 types of galactosylceramide (GalCer) obtained from bovine brain. Structural analysis of the 3 glycolipids revealed that they consisted of the same galactose and sphingosine but of apparently different fatty acids. Among the GalCers, the mAb reacted with teh two GalCers which contained alpha-hydroxy fatty acids, but not with GalCer composed of nonhydroxy fatty acids. These findings suggest that not only that the mAb discriminated the fatty acid composition in the ceramide moiety of GalCer, but also that the ceramide structure defines the immunological epitope as it is known to do for the carbohydrate moiety of glycosphingolipid.
H Nakakuma; M Arai; T Kawaguchi; K Horikawa; M Hidaka; K Sakamoto; M Iwamori; Y Nagai; K Takatsuki
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEBS letters     Volume:  258     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  1989 Dec 
Date Detail:
Created Date:  1990-01-29     Completed Date:  1990-01-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  230-2     Citation Subset:  IM    
Second Department of Internal Medicine, Kumamoto University Medical School, Japan.
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MeSH Terms
Antibodies, Monoclonal*
Antigen-Antibody Complex / analysis
Brain Chemistry
Carbohydrates / analysis
Ceramides / immunology*
Cerebrosides / immunology*
Chromatography, Thin Layer
Epitopes / analysis
Fatty Acids / analysis
Galactosylceramides / immunology*,  isolation & purification
Mice, Inbred BALB C / immunology
Pancreatic Neoplasms / immunology
Tumor Cells, Cultured / immunology
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antigen-Antibody Complex; 0/Carbohydrates; 0/Ceramides; 0/Cerebrosides; 0/Epitopes; 0/Fatty Acids; 0/Galactosylceramides

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