| Monoclonal antibody to galactosylceramide: discrimination of structural difference in the ceramide moiety. | |
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MedLine Citation:
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PMID: 2480915 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A mouse monoclonal antibody (mAb) was developed against monohexaosylceramide. This mAb differentially reacted on thin-layer chromatograms with 3 types of galactosylceramide (GalCer) obtained from bovine brain. Structural analysis of the 3 glycolipids revealed that they consisted of the same galactose and sphingosine but of apparently different fatty acids. Among the GalCers, the mAb reacted with teh two GalCers which contained alpha-hydroxy fatty acids, but not with GalCer composed of nonhydroxy fatty acids. These findings suggest that not only that the mAb discriminated the fatty acid composition in the ceramide moiety of GalCer, but also that the ceramide structure defines the immunological epitope as it is known to do for the carbohydrate moiety of glycosphingolipid. |
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Authors:
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H Nakakuma; M Arai; T Kawaguchi; K Horikawa; M Hidaka; K Sakamoto; M Iwamori; Y Nagai; K Takatsuki |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: FEBS letters Volume: 258 ISSN: 0014-5793 ISO Abbreviation: FEBS Lett. Publication Date: 1989 Dec |
Date Detail:
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Created Date: 1990-01-29 Completed Date: 1990-01-29 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0155157 Medline TA: FEBS Lett Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 230-2 Citation Subset: IM |
Affiliation:
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Second Department of Internal Medicine, Kumamoto University Medical School, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies, Monoclonal* Antigen-Antibody Complex / analysis Brain Chemistry Carbohydrates / analysis Ceramides / immunology* Cerebrosides / immunology* Chromatography, Thin Layer Epitopes / analysis Fatty Acids / analysis Galactosylceramides / immunology*, isolation & purification Humans Mice Mice, Inbred BALB C / immunology Pancreatic Neoplasms / immunology Rats Tumor Cells, Cultured / immunology |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/Antigen-Antibody Complex; 0/Carbohydrates; 0/Ceramides; 0/Cerebrosides; 0/Epitopes; 0/Fatty Acids; 0/Galactosylceramides |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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