Document Detail


Monoclonal antibody to cardiac myosin: imaging of experimental myocardial infarction.
MedLine Citation:
PMID:  6469268     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Monoclonal antibody R11D10 to human cardiac myosin, which also cross-reacted with canine cardiac myosin, was used to demonstrate in vivo localization and visualization by gamma scintigraphy of experimental myocardial infarction. R11D10 Fab with a Ka of 5 X 10(8) M-1 was labeled with technetium-99m (99mTc) by the dithionite reduction method of technetium pertechnetate, via a bifunctional chelating agent, diethylene triamine pentaacetic acid (DTPA). Uptake of 99mTc R11D10 Fab in the infarct can be visualized as early as 2 h after intravenous administration. Comparison of R11D10 uptake to thallium-201, an analogue of potassium which is sequestered by normal myocardium, showed an inverse relation (r = -0.75, -0.87, -0.89), similar to that obtained with 125I labeled polyclonal antimyosin Fab. Ratios of R11D10 Fab in the infarct to normal myocardium were as high as 30:1 where access of antibody to antigen was not blood flow limited. However, with severe blood-flow restriction, the ratios were lower at about 10:1. Despite the theoretical limitation of a single epitope per myosin molecule available for binding by R11D10 Fab, the immense excess of myosin in the infarcted myocardium allowed adequate concentration of radiolabeled R11D10 for visualization of the infarct by external gamma scintiscanning.
Authors:
B A Khaw; J A Mattis; G Melincoff; H W Strauss; H K Gold; E Haber
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Hybridoma     Volume:  3     ISSN:  0272-457X     ISO Abbreviation:  Hybridoma     Publication Date:  1984  
Date Detail:
Created Date:  1984-10-24     Completed Date:  1984-10-24     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8202424     Medline TA:  Hybridoma     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  11-23     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Monoclonal / immunology*
Antibody Affinity
Antibody Specificity
Dogs
Female
Humans
Immunoglobulin Fab Fragments / immunology
Male
Mice
Myocardial Infarction / radionuclide imaging*
Myosins / immunology*
Pentetic Acid
Technetium
Grant Support
ID/Acronym/Agency:
HL 26215/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Immunoglobulin Fab Fragments; 67-43-6/Pentetic Acid; 7440-26-8/Technetium; EC 3.6.4.1/Myosins

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