Document Detail


Monoclonal antibody neutralization of BPV-1.
MedLine Citation:
PMID:  7686316     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Mouse monoclonal antibodies were generated against intact infectious BPV-1 virions by methods previously described (Christensen et al., 1990). ELISA was used to screen for reactivities to intact and/or disrupted BPV-1, CRPV and HPV-11 virions. Several hybridomas were initially selected that showed antibody reactivity by ELISA to both intact and disrupted BPV-1, to disrupted BPV-1 only, or to intact BPV-1 virions. One monoclonal antibody, designated B1.A1, which reacted only to intact BPV-1 was selected for virus neutralization analyses. ELISA demonstrated that this monoclonal antibody bound to intact BPV-1 virions, but not to intact CRPV, HPV-11 or to disrupted papillomavirus (PV) antigens. Strong neutralization of BPV-1-induced focus formation of mouse C127 cells by monoclonal B1.A1 was observed. The neutralization titer was equivalent to the neutralization titer obtained with a polyclonal rabbit anti-BPV-1 virion antisera, and directly correlated with antibody concentration as determined by ELISA. These results extend our previous analyses on the epitopes of infectious papillomaviruses as defined by monoclonal antibodies that identify neutralizing epitopes. The nature of these epitopes is such that maintenance of the quaternary structure of the infectious virions is necessary for preservation of the antigenicity of the neutralizing epitope.
Authors:
N D Christensen; J W Kreider
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Virus research     Volume:  28     ISSN:  0168-1702     ISO Abbreviation:  Virus Res.     Publication Date:  1993 May 
Date Detail:
Created Date:  1993-07-23     Completed Date:  1993-07-23     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8410979     Medline TA:  Virus Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  195-202     Citation Subset:  IM    
Affiliation:
Department of Pathology, Milton S. Hershey Medical Center, Hershey, PA 17033.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Monoclonal / immunology
Antibodies, Viral / immunology
Bovine papillomavirus 1 / immunology*
Epitopes / immunology*
Mice
Neutralization Tests
Grant Support
ID/Acronym/Agency:
CA47622/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antibodies, Viral; 0/Epitopes

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