Document Detail

Monoclonal antibody HISL-19 as an immunocytochemical probe for neuroendocrine differentiation. Its application in diagnostic pathology.
MedLine Citation:
PMID:  3515956     Owner:  NLM     Status:  MEDLINE    
The monoclonal islet cell antibody HISL-19 was generated after immunization of BALB/c mice with human islet cell preparations. Besides reactivity with all cells of the human pancreatic islet, MAb HISL-19 also reacted with other cells of the diffuse neuroendocrine system, including anterior pituitary cells, C cells of the thyroid, endocrine cells of the gut and bronchus, the adrenal medulla, and central and peripheral neurons. In this study the authors screened a series of 53 neuroendocrine and 71 nonneuroendocrine tumors for their reactivity with MAb HISL-19 using an indirect immunoperoxidase technique on formalin-fixed and Paraplast-embedded sections. MAb HISL-19 reacted strongly with all insulomas (10), carcinoids (8), C-cell carcinomas of the thyroid (8), pituitary adenomas (6), neuroendocrine carcinomas of the skin (4), paragangliomas of the carotid body (3), and pheochromocytomas (2) tested. Neuroblastomas (3), oat-cell carcinomas of the lung (2), and melanomas (4) exhibited only very few immunoreactive cells scattered throughout the tumor or remained unstained with MAb HISL-19. With the exception of one lobular carcinoma of the breast (1/3), one adenocarcinoma of the endometrium (1/4), and one adenocarcinoma of the stomach (1/6), nonneuroendocrine tumors were negative with MAb HISL-19. Biochemical findings obtained by SDS-PAGE, "Western" immunoblotting, immunoaffinity chromatography, and absorption experiments indicate that the MAb HISL-19-defined antigen is not related to neuron specific enolase. Because the epitope recognized by MAb HISL-19 is well preserved in formalin-fixed and routinely processed tissues, this monoclonal antibody finds potential applications in diagnostic pathology as an indicator for neuroendocrine cells and their neoplasms.
K Krisch; P Buxbaum; G Horvat; I Krisch; N Neuhold; W Ulrich; S Srikanta
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The American journal of pathology     Volume:  123     ISSN:  0002-9440     ISO Abbreviation:  Am. J. Pathol.     Publication Date:  1986 Apr 
Date Detail:
Created Date:  1986-05-01     Completed Date:  1986-05-01     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0370502     Medline TA:  Am J Pathol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  100-8     Citation Subset:  AIM; IM    
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MeSH Terms
Antibodies, Monoclonal / immunology
Antigens, Neoplasm / analysis*
Chromatography, Affinity
Electrophoresis, Polyacrylamide Gel
Endocrine System Diseases / immunology*
Immunoenzyme Techniques
Insulinoma / immunology
Islets of Langerhans / immunology*
Neoplasms / immunology*
Nervous System Neoplasms / immunology*
Neurons / immunology
Pancreatic Neoplasms / immunology
Phosphopyruvate Hydratase / immunology
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antigens, Neoplasm; EC Hydratase

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