Document Detail

Monoclonal antibodies for the structural analysis of the Na+/H+ antiporter NhaA from Escherichia coli.
MedLine Citation:
PMID:  12586378     Owner:  NLM     Status:  MEDLINE    
Since their advent some 25 years ago, monoclonal antibodies have developed into powerful tools for structural and functional analysis of their cognate antigens. Together with the respective antigen binding fragments, antibodies offer exclusive capacities in detection, characterization, purification and functional assays for every given ligand. Antibody-fragment mediated crystallization represents a major advance in determining the three-dimensional structure of membrane-bound protein complexes. In this review, we focus on the methods used to generate monoclonal antibodies against the NhaA antiporter from Escherichia coli as a paradigm of secondary transporters. We describe examples on how antibodies are helpful in understanding structure and function relationships for this important class of integral membrane proteins. The generated conformation-specific antibody fragments are highly valuable reagents for co-crystallization attempts and structure determination of the antiporter.
Miro Venturi; Carola Hunte
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1610     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  2003 Feb 
Date Detail:
Created Date:  2003-02-14     Completed Date:  2003-06-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  46-50     Citation Subset:  IM    
Copyright Information:
Copyright 2003 Elsevier Science B.V.
Structural Biology Section, Vaccine Research Center/National Institutes of Health, Bethesda, MD 20892, USA.
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MeSH Terms
Antibodies, Monoclonal / chemistry*,  genetics,  immunology
Antibody Affinity
Cloning, Molecular
Epitopes / chemistry
Escherichia coli Proteins / biosynthesis*,  isolation & purification
Immunoglobulin Fab Fragments / chemistry
Immunoglobulin Fragments / chemistry
Membrane Proteins / biosynthesis*,  isolation & purification
Protein Conformation
Sodium-Hydrogen Antiporter / biosynthesis*,  immunology,  isolation & purification
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Epitopes; 0/Escherichia coli Proteins; 0/Immunoglobulin Fab Fragments; 0/Immunoglobulin Fragments; 0/Membrane Proteins; 0/NhaA protein, E coli; 0/Sodium-Hydrogen Antiporter

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