Document Detail


Monoclonal antibodies against surface antigens of Bacteroides gingivalis.
MedLine Citation:
PMID:  4044037     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Monoclonal antibodies against the various surface antigens of Bacteroides gingivalis were obtained by the fusion of murine myeloma cells (SP2/0-Ag14) with spleen cells of BALB/c mice immunized with the whole cells. Two monoclonal antibodies reacted with lipopolysaccharide, and the other two reacted strongly with capsule antigen. One showed reactivity with the hemagglutinin of the cells. The five monoclonal antibodies reacted with sonicated antigen from all B. gingivalis strains tested. No cross-reactivity of the monoclonal antibodies with antigens from nine species of other black-pigmented Bacteroids strains was observed. An immunoblotting test involving the use of these monoclonal antibodies indicated that the epitope of B. gingivalis lipopolysaccharide was polysaccharide with a high molecular weight of 40,000 to 60,000. The immunoblotting test also demonstrated that the epitopes of capsule antigen and of hemagglutinin were 27,000- and 40,000-molecular-weight proteins, respectively.
Authors:
Y Naito; K Okuda; T Kato; I Takazoe
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Infection and immunity     Volume:  50     ISSN:  0019-9567     ISO Abbreviation:  Infect. Immun.     Publication Date:  1985 Oct 
Date Detail:
Created Date:  1985-10-29     Completed Date:  1985-10-29     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  231-5     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Antibodies, Bacterial / immunology
Antibodies, Monoclonal / immunology*
Antibody Specificity
Antigens, Bacterial / immunology*
Antigens, Surface / immunology*
Bacterial Proteins / immunology
Bacteroides / immunology*
Hemagglutinins / immunology
Lipopolysaccharides / immunology
Molecular Weight
Polysaccharides, Bacterial / immunology
Chemical
Reg. No./Substance:
0/Antibodies, Bacterial; 0/Antibodies, Monoclonal; 0/Antigens, Bacterial; 0/Antigens, Surface; 0/Bacterial Proteins; 0/Hemagglutinins; 0/Lipopolysaccharides; 0/Polysaccharides, Bacterial
Comments/Corrections

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