Document Detail


Monoclonal antibodies against human esophageal carcinoma cell lines.
MedLine Citation:
PMID:  2827967     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Two monoclonal antibodies, designated 4C4 and 4G1, were produced by immunization of BALB/c mice with a human esophageal carcinoma cell line, CE69T/VGH, followed by fusion of the spleen cells from an immunized mouse with myeloma cells NS-1. 4C4 showed strong binding activity to three human esophageal carcinoma cell lines and one human hepatoma cell line, but not to any other cell lines tested. 4G1 reacted with three human esophageal carcinoma cell lines and four other cell lines. By peroxidase-antiperoxidase staining, 4C4 and 4G1 detected antigens of the epithelial cells on 10 pairs of esophageal carcinoma and normal esophageal specimens. 4G1 recognized a CE69T/VGH antigen with a molecular weight of 180K. Since 4G1 also reacted with purified carcinoembryonic antigen (CEA) and immunoprecipitated 125I-CEA, 4G1 seems to be an antibody recognizing CEA produced by CE69T/VGH cells. Since 4C4 also bound to the epithelial cells of normal uterine, vaginal, breast and liver tissues, it seems to recognize an epithelial antigen, and can be used to characterize the antigen in the specialization or differentiation of epithelial cells.
Authors:
H G Hsieh; C P Hu; S H Han; L R Chen; K Y Chien; C K Chou; L P Ting; T S Su; M H Huang; W H Hsu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Zhonghua Minguo wei sheng wu ji mian yi xue za zhi = Chinese journal of microbiology and immunology     Volume:  20     ISSN:  0253-2662     ISO Abbreviation:  Zhonghua Min Guo Wei Sheng Wu Ji Mian Yi Xue Za Zhi     Publication Date:  1987 Aug 
Date Detail:
Created Date:  1988-03-10     Completed Date:  1988-03-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8008067     Medline TA:  Zhonghua Min Guo Wei Sheng Wu Ji Mian Yi Xue Za Zhi     Country:  TAIWAN    
Other Details:
Languages:  eng     Pagination:  179-89     Citation Subset:  IM    
Affiliation:
Department of Medical Research, Veterans General Hospital, Taipei, ROC.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Monoclonal / immunology*
Antibodies, Neoplasm / immunology*
Antigens, Neoplasm / isolation & purification
Carcinoembryonic Antigen / immunology
Carcinoma, Hepatocellular / immunology
Cell Line
Epithelium / immunology
Esophageal Neoplasms / immunology*
Humans
Liver Neoplasms / immunology
Mice
Molecular Weight
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antibodies, Neoplasm; 0/Antigens, Neoplasm; 0/Carcinoembryonic Antigen

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