Document Detail


Monoclonal antibodies against human BAP31 for immunocytochemistry.
MedLine Citation:
PMID:  19519245     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Human BAP31 is a 28 kDa polytopic integral protein of the ER and part of a large BAP hetero-oligomeric complex that includes the related BAP29 protein and connections to actomyosin. BAP31 interacts with mIgD, cellubrevin, major histocompatibility complex class I, and BCL-2/BCL-X(L), and plays an important role in regulating the egress of these proteins and in apoptosis. Northern blot analyses have revealed BAP31 RNA transcripts in many tissues, including thymus, spleen, brain, kidney, testis, liver, and lung. However, prominent BAP31 protein expression analyzed by immunohistochemistry is restricted to a minority of cells in normal human tissue. Further studies should be made to verify the expression profiles of BAP31 in the protein level. Production of high affinity MAbs suitable for immunohistochemical staining has lagged. Here we generate a set of MAbs that could be used in Western blot, immunoprecipitation, and immunocytochemistry, providing a new powerful tool for investigation of expression profile of BAP31 protein and furthers the study of BAP31 functions.
Authors:
Chaojun Song; Fuli Wang; Zhuwei Xu; Jintao Hu; Haiqiang Tao; Angang Yang; Kun Yang; Boquan Jin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Hybridoma (2005)     Volume:  28     ISSN:  1557-8348     ISO Abbreviation:  Hybridoma (Larchmt)     Publication Date:  2009 Jun 
Date Detail:
Created Date:  2009-06-12     Completed Date:  2009-09-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101241539     Medline TA:  Hybridoma (Larchmt)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  177-81     Citation Subset:  IM    
Affiliation:
Department of Immunology, The Fourth Military Medical University, Xi'an, P.R. China. scj0530@yahoo.com.cn
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Monoclonal / immunology*
Blotting, Northern
Blotting, Western
Enzyme-Linked Immunosorbent Assay
Female
Humans
Immunohistochemistry
Membrane Proteins / genetics,  immunology*,  isolation & purification,  metabolism
Mice
Mice, Inbred BALB C
Recombinant Proteins / genetics,  immunology,  isolation & purification,  metabolism
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/BCAP31 protein, human; 0/Membrane Proteins; 0/Recombinant Proteins

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