Document Detail

Monoclonal anti-allotype antibody towards BALB/c IgM. Analysis of specificity and site of a V-C crossover in recombinant strain BALB-Igh-Va/Igh-Cb.
MedLine Citation:
PMID:  3582489     Owner:  NLM     Status:  MEDLINE    
Polyclonal IgM obtained by fractionation of a BALB/c serum pool was used as the immunogen for C57BL/6 mice. Draining lymph nodes from selected animals donated cells for fusion with myeloma Sp-2/0. Fifteen hybridomas were productive, and one (RS-3.1) was cloned and the affinity-purified product analyzed for its reactivity pattern by MOPC 104E-enzyme-linked immunosorbent assay inhibition. Among five BALB/c myelomas only TEPC 183 (IgM) was active, not those belonging to other Ig classes. Among normal sera from 8 mouse strains only those of BALB/c, DBA/2J and CBA/J showed inhibition. The recombinant strain BALB-Igh-Va/Igh-Cb did not react, which shows that its C mu stems from parental strain C57BL/6, and that therefore the recombination event had occurred 5' of this gene.
R Schüppel; J Wilke; E Weiler
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  European journal of immunology     Volume:  17     ISSN:  0014-2980     ISO Abbreviation:  Eur. J. Immunol.     Publication Date:  1987 May 
Date Detail:
Created Date:  1987-07-23     Completed Date:  1987-07-23     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  1273201     Medline TA:  Eur J Immunol     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  739-41     Citation Subset:  IM    
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MeSH Terms
Antibodies, Monoclonal / immunology*
Antibody Specificity
Immunoglobulin Allotypes / immunology*
Immunoglobulin Constant Regions / genetics,  immunology
Immunoglobulin M / immunology*
Immunoglobulin Variable Region / genetics,  immunology
Mice, Inbred BALB C / immunology*
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Immunoglobulin Allotypes; 0/Immunoglobulin Constant Regions; 0/Immunoglobulin M; 0/Immunoglobulin Variable Region

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