Document Detail

Monitoring structural changes in cells with high-frequency ultrasound signal statistics.
MedLine Citation:
PMID:  16085095     Owner:  NLM     Status:  MEDLINE    
We investigate the use of signal envelope statistics to monitor and quantify structural changes during cell death using an in vitro cell model. Using a f/2.35 transducer (center frequency 20 MHz), ultrasound backscatter data were obtained from pellets of acute myeloid leukemia cells treated with a DNA-intercolating chemotherapy drug, as well as from pellets formed with mixtures of treated and untreated cells. Simulations of signals from pellets of mixtures of cells were generated as a summation of point scatterers. The signal envelope statistics were examined by fitting the Rayleigh and generalized gamma distributions. The fit parameters of the generalized gamma distribution showed sensitivity to structural changes in the cells. The scale parameter showed a 200% increase (p<0.05) between untreated and cells treated for 24 h. The shape parameter showed a 50% increase (p<0.05) over 24 h. Experimental results showed reasonable agreement with simulations. The results indicate that high-frequency ultrasound signal statistics can be used to monitor structural changes within a very low percentage of treated cells in a population, raising the possibility of using this technique in vivo.
A S Tunis; G J Czarnota; A Giles; M D Sherar; J W Hunt; M C Kolios
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Ultrasound in medicine & biology     Volume:  31     ISSN:  0301-5629     ISO Abbreviation:  Ultrasound Med Biol     Publication Date:  2005 Aug 
Date Detail:
Created Date:  2005-08-08     Completed Date:  2006-03-28     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0410553     Medline TA:  Ultrasound Med Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1041-9     Citation Subset:  IM    
Department of Medical Biophysics, University of Toronto, Toronto, ONT, Canada.
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MeSH Terms
Antineoplastic Agents / pharmacology
Apoptosis / drug effects,  physiology*
Cell Nucleus / physiology,  ultrasonography
Cisplatin / pharmacology
Computer Simulation
Leukemia, Myeloid, Acute / physiopathology,  ultrasonography*
Models, Biological
Monitoring, Physiologic / methods
Time Factors
Tumor Cells, Cultured
Reg. No./Substance:
0/Antineoplastic Agents; 15663-27-1/Cisplatin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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